PMID: 8471548Jan 1, 1993Paper

Experience with the PCR-based HLA-DQ alpha DNA typing system in routine forensic casework

International Journal of Legal Medicine
P M Schneider, C Rittner

Abstract

The results of HLA-DQ alpha typing from 42 routine forensic cases using the polymerase chain reaction (PCR) were analyzed regarding the reliability, discrimination efficiency and informative value of this system in a given case. The cases included stain typing from a variety of different substates, i.e. blood and semen stains, mixed body fluids, single hairs, cigarette butts, material from fingernail scratches, as well as identification and paternity cases on postmortem and fixed tissue. A total of 125 individual stain and tissue samples were included. PCR amplification was achieved in 70% of these samples. In cases with mixed body fluids, e.g. sperm and vaginal cells from rape cases, DQ alpha typing was always carried out successfully. However, only approx. 42% of all samples that could be typed were relevant regarding the inclusion or exclusion of a suspect. This was mostly due to the limited number of alleles that can be typed at the HLA-DQ alpha locus or to the fact that the stain or hair samples did not originate from the perpetrator, but from the victim or from other persons not related to the crime.

References

Jan 1, 1992·International Journal of Legal Medicine·S RandB Brinkmann
Jan 1, 1990·Zeitschrift für Rechtsmedizin. Journal of legal medicine·M OgataC Rittner
Apr 1, 1990·Forensic Science International : Synergy·S A Westwood, D J Werrett
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R K SaikiH A Erlich
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E BoerwinkleL Chan
Apr 7, 1988·Nature·R HiguchiH A Erlich
Dec 12, 1985·Nature·P GillD J Werrett

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Citations

May 9, 1995·Forensic Science International : Synergy·J B CowlandN Morling
Jul 31, 1996·Forensic Science International : Synergy·N Dimo-Simonin, C Brandt-Casadevall
Mar 11, 1998·Forensic Science International : Synergy·E Lachica, R García-Ferrer
May 26, 2007·Journal of Forensic Sciences·Jessica V NorrisJames P Landers
Jun 1, 1997·Journal of Clinical Forensic Medicine·T KondoB Bayer
Jan 12, 2020·Science & Justice : Journal of the Forensic Science Society·Agnieszka KuffelNiamh Nic Daeid

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