Experimental and computational assessment of F-actin influence in regulating cellular stiffness and relaxation behaviour of fibroblasts

Journal of the Mechanical Behavior of Biomedical Materials
B FallqvistAnnica K B Gad

Abstract

In biomechanics, a complete understanding of the structures and mechanisms that regulate cellular stiffness at a molecular level remain elusive. In this paper, we have elucidated the role of filamentous actin (F-actin) in regulating elastic and viscous properties of the cytoplasm and the nucleus. Specifically, we performed colloidal-probe atomic force microscopy (AFM) on BjhTERT fibroblast cells incubated with Latrunculin B (LatB), which results in depolymerisation of F-actin, or DMSO control. We found that the treatment with LatB not only reduced cellular stiffness, but also greatly increased the relaxation rate for the cytoplasm in the peripheral region and in the vicinity of the nucleus. We thus conclude that F-actin is a major determinant in not only providing elastic stiffness to the cell, but also in regulating its viscous behaviour. To further investigate the interdependence of different cytoskeletal networks and cell shape, we provided a computational model in a finite element framework. The computational model is based on a split strain energy function of separate cellular constituents, here assumed to be cytoskeletal components, for which a composite strain energy function was defined. We found a significant influence...Continue Reading

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Citations

Oct 21, 2016·Biophysical Journal·Tom BongiornoTodd Sulchek
Jan 10, 2018·Advanced Materials·Dedy SeptiadiAlke Petri-Fink
Sep 27, 2018·Cells·Frida DanielssonAnnica Karin Britt Gad
Dec 10, 2016·Cell Biochemistry and Biophysics·Michele MenottaMauro Magnani
Dec 2, 2018·Frontiers in Physiology·Francesco BasoliAlberto Rainer
Jun 27, 2019·Scientific Reports·Camille FuselierChristophe Schneider
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Aug 17, 2021·Computer Methods in Biomechanics and Biomedical Engineering·Sundeep Singh, Roderick Melnik
Nov 25, 2020··Alina MelikhovaIvan Melikhov

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