PMID: 15161851May 27, 2004

Experimental and numerical studies of adenovirus delivery to outflow tissues of perfused human anterior segments

Investigative Ophthalmology & Visual Science
C R EthierW D Stamer

Abstract

To investigate the efficacy of two different methods of adenoviral transfer of genes to trabecular meshwork (TM) and Schlemm's canal (SC) cells in cultured human anterior segments, using both experimental and numerical analyses. Replication-deficient adenoviruses having coding sequence for beta-galactosidase (beta-gal) under the control of the cytomegalovirus promoter were used. Efficiency of gene transfer over time was verified by infecting cultured human TM cells and assaying for beta-gal activity. Next, ostensibly normal paired human eyes were prepared by standard techniques and perfused for 2 to 5 days to measure baseline facilities. Eyes were then infected by one of two methods: standard transcorneal puncture, or injection into a 1 mm diameter silastic segment of supply tubing immediately upstream of the perfusion dish. In both cases, the nominal total dose was 2 x 10(8) viral particles. Five days after viral injection, eyes were harvested and fixed, and wedges from each of four quadrants were examined histologically. Sections were assayed for beta-gal activity and/or stained with toluidine blue. In a parallel study, flow and viral transport within perfused anterior segments were numerically simulated for conditions that a...Continue Reading

Citations

Apr 20, 2013·Investigative Ophthalmology & Visual Science·Dong-Jin OhDouglas J Rhee
Feb 12, 2014·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Zeynep AktasCarol A Rasmussen
Jul 29, 2008·Experimental Eye Research·W D StamerC R Ethier
Jul 28, 2013·Translational Vision Science & Technology·Baohe Tian, Paul L Kaufman
May 2, 2016·Experimental Eye Research·Yalong DangNils A Loewen
Apr 11, 2018·American Journal of Physiology. Cell Physiology·Fiona McDonnellW D Stamer
Mar 18, 2020·Investigative Ophthalmology & Visual Science·Ajay AshokNeena Singh

Related Concepts

Adenoviruses, Human
Anterior Eyeball Segment Structure
Lactogest
In Silico
Defective Viruses
Shuttle Vectors
Injectables
Organ Culture Techniques
Trabecular Meshwork Structure
Gene Expression Regulation, Enzymologic

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