Experimental and theoretical study of selective protein deposition using focused micro laminar flows

Biomedical Microdevices
Avishay BranskyShulamit Levenberg

Abstract

The present work describes an experimental method and design tools which enable the precise localization of an analyte, a few microns in width, both temporally and spatially using laminar flows and thus improves previous methods in hydrodynamic focusing. The technique is used to adsorb proteins to selected regions within a microfluidic device without any contamination of the surroundings and may serve in applications requiring selective conveying of other reagents. The regions not coated by proteins are modified with poly(ethylene glycol; PEG), known to efficiently resist protein and cell adhesion. Human endothelial and fibroblast cells are later introduced into the device selectively attaching to the protein coated regions and cultured for a few days. A simulation of the convection-diffusion characteristics of the system is presented and compared to the known T-sensor. The results reveal that, by proper design, reagents concentration may be kept nearly constant along the flow direction. This phenomenon is demonstrated here by achieving particularly precise patterning of cells but may be utilized for numerous other applications as well.

References

May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·S TakayamaG M Whitesides
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·J SatulovskyI Szleifer
Aug 22, 2000·Journal of Biomedical Materials Research·A FolchM Toner
Dec 22, 2000·Accounts of Chemical Research·P J KenisH S White
Jun 29, 2001·Nature·S TakayamaG M Whitesides
Jun 19, 2002·Analytical Chemistry·Anne Y FuStephen R Quake
Jul 16, 2002·Annual Review of Biomedical Engineering·David J BeebeGlenn M Walker
Aug 27, 2002·Developmental Cell·Asako SawanoAtsushi Miyawaki
Mar 6, 2003·Chemistry & Biology·Shuichi TakayamaGeorge M Whitesides
Dec 1, 2004·Lab on a Chip·Birgitte RegenbergThomas Ahl
Dec 24, 2004·Lab on a Chip·Anna TourovskaiaAlbert Folch
Dec 24, 2004·Lab on a Chip·Seog Woo RheeNoo Li Jeon
Dec 1, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Seunghwan Lee, Janos Vörös
Nov 17, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Rodolphe MarieFredrik Höök
Dec 6, 2006·Biomedical Microdevices·Ali KhademhosseiniMilica Radisic
Jan 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Chia-Ching WuShu Chien
May 4, 2007·Lab on a Chip·Natanel KorinShulamit Levenberg

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Citations

Oct 24, 2013·Analytical and Bioanalytical Chemistry·Mark D TarnNicole Pamme
Jul 29, 2010·Biomedical Microdevices·Maria KhouryShulamit Levenberg
Dec 18, 2013·Biomedical Microdevices·Xuan MuGuoan Luo
Dec 16, 2008·IEEE Reviews in Biomedical Engineering·Jay W WarrickDavid J Beebe
Jan 13, 2010·Lab on a Chip·Edmond W K Young, Craig A Simmons
Feb 5, 2009·Lab on a Chip·Avishay BranskyShulamit Levenberg
Sep 9, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·E Corvera Poiré, A Hernández-Machado

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