Experimental approach for a possible integrated protocol to determine sanitizer activity against both planktonic bacteria and related biofilms

Food Research International
Raffaella CampanaWally Baffone

Abstract

The persistence of pathogenic bacteria in industrial settings is linked to biofilm embedded bacteria resistance to antimicrobial and disinfectant methods effective against planktonic cells. We proposed an experimental approach to evaluate sanitizers effectiveness against both planktonic microorganisms and related biofilms as possible integration of the official EN 1276 procedure. Firstly, the efficacy of three chemicals sanitizers was tested on planktonic cells of Escherichia coli O157:H7 ATCC 35150, Staphylococcus aureus ATCC 43387, Pseudomonas aeruginosa ATCC 9027, Enterococcus faecalis ATCC 29212 and Candida albicans ATCC 14053 using the suspension test indicated by EN 1276 in both dirty and clear simulated conditions (0.3% or 0.03% of bovine serum albumen). The sanitizers were tested against the related biofilms developed on stainless steel for 48 h at room temperature. The sanitizers (SANI 626, SUPERIG, IGIEN 155) reached 5-logarithmic reduction at the manufacture's recommended concentrations after 30 s and 5 min against planktonic microorganisms but, sometimes, the organic load interfered with their activity. The same concentrations tested with the proposed protocol weren't effective against biofilms and a log reduction >...Continue Reading

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