Experimental Autoimmune Encephalomyelitis Is Successfully Controlled by Epicutaneous Administration of MOG Plus Vitamin D Analog

Frontiers in Immunology
Sofia F G Zorzella-PezaventoAlexandrina Sartori

Abstract

Multiple sclerosis (MS) is an inflammatory and demyelinating disorder of the central nervous system (CNS). Experimental autoimmune encephalomyelitis (EAE) has been widely employed to evaluate new strategies to control MS, including procedures to induce immunological tolerance. Considering that skin exposure to protein antigens can induce tolerance and that vitamin D analogs conserve immunomodulatory potential and are less toxic, we investigated the efficacy of epicutaneous application of a myelin oligodendrocyte glycoprotein peptide (MOG35-55) associated with paricalcitol (PARI) on EAE development. Three and 11 days after EAE induction, C57BL/6 mice were treated with an occlusive patch containing MOG plus PARI. Clinical parameters were daily assessed, whereas immunological and histological evaluations were performed during the acute EAE phase. MOG and MOG + PARI significantly controlled disease development reducing weight loss and clinical score. Moreover, MOG and MOG + PARI reduced the inflammatory process and preserved the myelin sheath in the CNS. High percentages of Foxp3(+) regulatory T cells (Tregs) and lower MHCII fluorescence intensity in dendritic cells in draining lymph nodes were concomitantly observed. MOG + PARI as...Continue Reading

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Citations

May 2, 2020·International Journal of Molecular Sciences·Samiksha WasnikXiaolei Tang
May 13, 2020·Neural Regeneration Research·Karen Henriette PinkeAlexandrina Sartori
Aug 30, 2019·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Karen Henriette PinkeAlexandrina Sartori
Apr 1, 2020·Frontiers in Pharmacology·Larissa Ragozo Cardoso de OliveiraAlexandrina Sartori
Nov 17, 2020·Frontiers in Immunology·Juliana Helena Dos Santos de ToledoSofia Fernanda Gonçalves Zorzella-Pezavento
Mar 7, 2021·International Journal of Molecular Sciences·Luiza Ayumi Nishiyama MimuraAlexandrina Sartori

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
FACS
flow cytometry
transmission electron microscopy
electron microscopy

Software Mentioned

SigmaPlot
FACS Diva Array
FlowJo

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