Experimental autoimmune encephalomyelitis: isolation and characterization of inflammatory cells from the central nervous system.

Journal of Neuroimmunology
W D LymanC S Raine

Abstract

Lymphocytes from the central nervous system (CNS) of animals with acute experimental autoimmune encephalomyelitis (EAE) have been isolated and characterized. The lymphocytes were separated from Lewis rats which were injected either with an emulsion of myelin basic protein (BP) emulsified in complete Freund's adjuvant (CFA) to cause EAE or with CFA alone as a control. Using density gradient centrifugation, from 9 days post-inoculation (d.p.i.) (before clinical signs appear), to 19 d.p.i. (after signs abate), lymphocytes were recovered from the spinal cords and popliteal lymph nodes of BP-injected animals. Lymphocyte cell number, phenotype, and antigen specificity were determined. Results show that the onset of clinical signs correlated with lymphocyte influx into the CNS. A clinical index of 1 was associated with less than 10(6) cells per gram of CNS wet weight (cells/g CNS) while animals with a clinical index of 4 had more than 15 X 10(6) cells/g CNS. During remission, when only minor residual neurologic signs were evident, significant numbers of lymphocytes (greater than 10(7) cells/g CNS) could still be isolated. In contrast, no lymphocytes were obtained from control CNS tissue. The phenotype of the recovered cells was predom...Continue Reading

References

Apr 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·A A VandenbarkR M Vetto
Jul 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·G A HashimL E Stevens
Feb 1, 1975·The Journal of Experimental Medicine·H M Wisniewski, B R Bloom
Jan 1, 1988·Journal of Neuroimmunology·W D LymanC S Raine
Jan 1, 1981·Immunological Reviews·P Y PatersonC C Whitacre
Jan 1, 1982·Annual Review of Neuroscience·G M McKhann

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