Experimental colitis is associated with ultrastructural changes in inflamed and uninflamed regions of the gastrointestinal tract

Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre
Azhar M Bou-FersenI Khan

Abstract

The objective of this study was to examine the ultrastructural changes in cell organelles such as mitochondria, endoplasmic reticulum (ER) and Golgi apparatus in inflamed colon and uninflamed ileum in colitic rats. Colitis was induced in rats by intracolonic administration of trinitrobenzenesulfonic acid (TNBS). The animals were sacrificed on day 5 after TNBS administration and colonic and ileal samples were used for estimation of myeloperoxidase (MPO) activity, malondialdehyde (MDA) concentration, histologic examination and transmission electron microscopy. TNBS caused a significant reduction in body weight and an increase in MPO activity in colonic, but not in the ileal samples in animals with colitis. MDA levels were increased both in inflamed colon and the uninflamed ileal segments in colitis. Electron microscopy revealed swelling of mitochondria with broken cristae and disruption of the inner membrane. Colitis also caused fragmentation of the ER with loss of ribosomes and swelling of the Golgi apparatus with distended vesicles in both smooth muscle and epithelial cells in the ileal and colonic segments. These changes were absent in the control rats without colitis. These findings demonstrate ultrastructural deformities in ...Continue Reading

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Citations

Jun 12, 2010·BMC Gastroenterology·Amir J GuriJosep Bassaganya-Riera
Oct 3, 2008·Scandinavian Journal of Gastroenterology·Asmaa S LubbadIslam Khan
Oct 9, 2015·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Purnima SharmaKishore Kumar Deepak
May 15, 2013·Journal of Veterinary Internal Medicine·D WalkerO A Garden
May 12, 2017·PloS One·Amal Ali SoleimanIslam Khan
Sep 28, 2010·Journal of the Science of Food and Agriculture·Ching L HiiSuzannah Sharif
May 10, 2019·Microscopy Research and Technique·Hiroki BochimotoMichihiro Fukushima
Apr 3, 2019·International Journal of Integrated Care·Patricia Sullivan-Taylor
Dec 15, 2015·International Journal of Cosmetic Science·A BialekA Tokarz
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Jul 31, 2018·Oxidative Medicine and Cellular Longevity·Ewa DudzińskaJanusz Kocki

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