Experimental control and characterization of autophagy in Drosophila.

Methods in Molecular Biology
Gabor Juhasz, Thomas P Neufeld

Abstract

Insects such as the fruit fly Drosophila melanogaster, which fundamentally reorganize their body plan during metamorphosis, make extensive use of autophagy for their normal development and physiology. In the fruit fly, the hepatic/adipose organ known as the fat body accumulates nutrient stores during the larval feeding stage. Upon entering metamorphosis, as well as in response to starvation, these nutrients are mobilized through a massive induction of autophagy, providing support to other tissues and organs during periods of nutrient deprivation. High levels of autophagy are also observed in larval tissues destined for elimination, such as the salivary glands and larval gut. Drosophila is emerging as an important system for studying the functions and regulation of autophagy in an in vivo setting. In this chapter we describe reagents and methods for monitoring autophagy in Drosophila, focusing on the larval fat body. We also describe methods for experimentally activating and inhibiting autophagy in this system and discuss the potential for genetic analysis in Drosophila to identify novel genes involved in autophagy.

Citations

Feb 20, 2009·Molecular Biology of the Cell·Yu-Yun Chang, Thomas P Neufeld
Dec 17, 2014·Proceedings of the National Academy of Sciences of the United States of America·Youheng WeiMary A Lilly
Nov 2, 2016·Autophagy·Anindita MukherjeeAndreas Jenny
Jan 15, 2016·Journal of Cell Science·Caroline MauvezinThomas P Neufeld
Aug 24, 2016·PloS One·Christie A BaderDouglas A Brooks
Feb 17, 2015·Nature Cell Biology·Yan-Ning RuiSheng Zhang
May 12, 2016·PLoS Genetics·Weili CaiMary A Lilly
Oct 8, 2016·The FEBS Journal·Christopher R Braden, Thomas P Neufeld
Jul 15, 2017·Cells·Péter LőrinczGábor Juhász
Mar 2, 2016·Parasites & Vectors·Hector Manuel Díaz-AlbiterFernando Ariel Genta
Sep 7, 2018·Molecular Biology of the Cell·Francesca Di CaraRichard A Rachubinski
Jun 20, 2020·Autophagy·Ana MesquitaAndreas Jenny
Sep 4, 2014·Cold Spring Harbor Protocols·Lindsay DeVorkin, Sharon M Gorski

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