PMID: 9179770Jun 1, 1997Paper

Experimental CVB3-induced chronic myocarditis in two murine strains: evidence of interrelationships between virus replication and myocardial damage in persistent cardiac infection

Journal of Medical Virology
L AndréolettiP Wattré

Abstract

In order to analyse the relationships between enteroviral replication and the myocardial damage at the onset of chronic cardiac infection, 2 mouse strains with different degrees of immunological competence (NMRI nu/nu, DBA/2) were infected by a myocarditic Coxsackie virus B3 (CVB3-M1) variant. At 31 days post-inoculation, plaque-forming assay, polymerase chain reaction (RT-PCR), and immunohistochemistry were carried out for detecting viruses and viral components in the myocardium. The virological findings were related to histopathological changes in the myocardium as well to the dilatation of both cardiac ventricles. Chronic myocardial lesions characterized by large fibrosis areas and interstitial inflammatory infiltrates were detected together with cardiomegalia in 52.6% (10/19) of athymic mice and in 9% (2/22) of euthymic mice. Viral replication foci were located and were found only in myocarditic cells adjacent to myocardial inflammatory lesions by immunostaining myocardial tissue sections with anti-serum to VP1 virus capsid protein. Using PCR followed by microwell capture hybridization assay, a large excess of viral positive strand RNA over negative strand was semiquantified in heart tissue from mice with chronic myocarditi...Continue Reading

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Citations

Apr 29, 2011·Journal of Virology·Outi PaloheimoMaija Vihinen-Ranta
Nov 9, 2000·The New England Journal of Medicine·A M Feldman, D McNamara
Mar 28, 2007·Heart Failure Clinics·Neil E BowlesJeffrey A Towbin
Apr 19, 2002·The Journal of General Virology·Sophie GirardBruno Blondel
Dec 20, 2007·The Journal of General Virology·Mónica HerreraEsteban Domingo
Aug 12, 2009·Archives of Cardiovascular Diseases·Laurent AndréolettiPaul Fornes
Oct 28, 2016·Virchows Archiv : an International Journal of Pathology·Nicole Ettischer-SchmidKarin Klingel
Sep 12, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Christine LangKarin Klingel
Jan 30, 2004·Journal of Medical Virology·Suvi RasilainenTimo Otonkoski
Sep 21, 2005·Circulation·Uwe KühlHeinz-Peter Schultheiss
Oct 5, 2018·Journal of Translational Medicine·Santa RasaUNKNOWN European Network on ME/CFS (EUROMENE)

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