Experimental Evaluation of the Density of Water in a Cell by Raman Microscopy

The Journal of Physical Chemistry Letters
Mizuki TakeuchiTakakazu Nakabayashi


We report direct observation of a spatial distribution of water molecules inside of a living cell using Raman images of the O-H stretching band of water. The O-H Raman intensity of the nucleus was higher than that of the cytoplasm, indicating that the water density is higher in the nucleus than that in the cytoplasm. The shape of the O-H stretching band of the nucleus differed from that of the cytoplasm but was similar to that of the balanced salt solution surrounding cells, indicating less crowded environments in the nucleus. The concentration of biomolecules having C-H bonds was also estimated to be lower in the nucleus than that in the cytoplasm. These results indicate that the nucleus is less crowded with biomolecules than the cytoplasm.


Aug 19, 2007·Nature Methods·Wonshik ChoiMichael S Feld
Oct 27, 2007·Biochimica Et Biophysica Acta·Jennifer L DashnauJane M Vanderkooi
Apr 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Erik Persson, Bertil Halle
Oct 19, 2010·Current Opinion in Biotechnology·Jacek T Mika, Bert Poolman
Oct 21, 2010·Biophysical Journal·Jinping DongAlptekin Aksan
Dec 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Masaya OkadaKatsumasa Fujita
Jan 23, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Dibyendu Kumar SasmalKankan Bhattacharyya
May 28, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Shirsendu GhoshKankan Bhattacharyya
Jul 16, 2013·Nature Nanotechnology·Cheemeng TanPhilip Leduc
May 9, 2014·The Journal of Physical Chemistry. B·Shyamtanu ChattorajKankan Bhattacharyya
Feb 6, 2015·Frontiers in Physiology·Miguel A Aon, Sonia Cortassa
Mar 25, 2016·Journal of Biophotonics·Mirjam SchürmannChii J Chan
Feb 22, 2017·Chemical Communications : Chem Comm·Hamid SoleimaninejadYuning Hong
May 11, 2017·Physical Chemistry Chemical Physics : PCCP·Md Asif AminKankan Bhattacharyya
May 19, 2017·The Journal of Physical Chemistry. B·Megan CurrieAhmed A Heikal

❮ Previous
Next ❯


Sep 29, 2020·Physical Chemistry Chemical Physics : PCCP·Hiroaki TakahashiTakakazu Nakabayashi
Feb 13, 2020·Angewandte Chemie·Toshiki SugimuraTakakazu Nakabayashi
Dec 16, 2020·Applied Spectroscopy·Michelle BaileyFrancesca Palombo
Mar 17, 2020·The Journal of Physical Chemistry. B·Youngjin ChoiMinhaeng Cho
Feb 2, 2018·The Journal of Physical Chemistry. B·Somen NandiKankan Bhattacharyya
Sep 29, 2020·Journal of Chemical Information and Modeling·Daisuke MiyataShin-Ichi Morita
Sep 15, 2021·The Journal of Physical Chemistry. B·Qi YangTakakazu Nakabayashi
Oct 10, 2021·Communications Biology·Masaaki Uematsu, Takao Shimizu

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.