Experimental Evaluation of the Density of Water in a Cell by Raman Microscopy

The Journal of Physical Chemistry Letters
Mizuki TakeuchiTakakazu Nakabayashi

Abstract

We report direct observation of a spatial distribution of water molecules inside of a living cell using Raman images of the O-H stretching band of water. The O-H Raman intensity of the nucleus was higher than that of the cytoplasm, indicating that the water density is higher in the nucleus than that in the cytoplasm. The shape of the O-H stretching band of the nucleus differed from that of the cytoplasm but was similar to that of the balanced salt solution surrounding cells, indicating less crowded environments in the nucleus. The concentration of biomolecules having C-H bonds was also estimated to be lower in the nucleus than that in the cytoplasm. These results indicate that the nucleus is less crowded with biomolecules than the cytoplasm.

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Citations

Sep 29, 2020·Physical Chemistry Chemical Physics : PCCP·Hiroaki TakahashiTakakazu Nakabayashi
Feb 13, 2020·Angewandte Chemie·Toshiki SugimuraTakakazu Nakabayashi
Dec 16, 2020·Applied Spectroscopy·Michelle BaileyFrancesca Palombo
Mar 17, 2020·The Journal of Physical Chemistry. B·Youngjin ChoiMinhaeng Cho
Feb 2, 2018·The Journal of Physical Chemistry. B·Somen NandiKankan Bhattacharyya
Sep 29, 2020·Journal of Chemical Information and Modeling·Daisuke MiyataShin-Ichi Morita
Sep 15, 2021·The Journal of Physical Chemistry. B·Qi YangTakakazu Nakabayashi
Oct 10, 2021·Communications Biology·Masaaki Uematsu, Takao Shimizu

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