PMID: 29783206May 22, 2018Paper

Experimental evaluation of the therapeutic potential of liposome-mediated apoe3 gene transfection for cerebral atherosclerosis

Wiadomości lekarskie : organ Polskiego Towarzystwa Lekarskiego
Vadym V BiloshytskyiOlha B Shevnia

Abstract

Introduction: In recent years, a stroke has been the cause of high lethality, long-term and sustained disability, the problem of which is still far from being resolved. The root cause of stroke is atherosclerosis. The aim: Study of neurotrophic and neuroprotective functions of apoE in rat brain tissue in experimental atherosclerosis. Materials and methods: The study included 60 sexually mature rats. The model of experimental atherosclerosis was created by Anichkov method. The experimental animals were divided into 3 groups: control, experimental and a group of animals with experimental atherosclerosis with intrapoly gene apolipoprotein E (apoE) 50 μg of DNA per animal injected on the 1st day of atherosclerosis modeling. Results: Morphological study of cortex in III and V layers in animals with experimental atherosclerosis showed a quantitative and qualitative changes in cellular composition. Neurons had atrophic and destructive changes as well as karyopicnose and carriolysis, nucleoli often were not imagined. Reduced number of normochromic cells (up to 50%), hypochromic cells (up to 4%), double increased number of hyperchromic neurons (up to 10%), prominently hyperchromic neurocytes (up to 20%) and prominently hypochromic neuro...Continue Reading

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