Experimental evolution to increase the efficacy of the entomopathogenic fungus Beauveria bassiana against malaria mosquitoes: Effects on mycelial growth and virulence

Evolutionary Applications
Claudio A Valero-JiménezSijmen E Schoustra

Abstract

Entomopathogenic fungi such as Beauveria bassiana are currently considered as a potential control agent for malaria mosquitoes. The success of such strategies depends among others on the efficacy of the fungus to kill its hosts. As B. bassiana can use various resources for growth and reproduction, increasing the dependency on mosquitoes as a nutritional source may be instrumental for reaching this goal. Passage of entomopathogenic fungi through an insect host has been shown to increase its virulence. We evaluated the virulence, fungal outgrowth, mycelial growth rate, and sporulation rate of two B. bassiana isolates (Bb1520 and Bb8028) that underwent 10 consecutive selection cycles through malaria mosquitoes (Anopheles coluzzii) using an experimental evolution approach. This cycling resulted in an altered capacity of evolved B. Bassiana lineages to grow on different substrates while maintaining the ability to kill insects. Notably, however, there were no significant changes in virulence or speed of outgrowth when comparing the evolved lineages against their unevolved ancestors. These results suggest that fungal growth and sporulation evolved through successive and exclusive use of an insect host as a nutritional resource. We dis...Continue Reading

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Citations

Sep 13, 2019·PLoS Pathogens·Matthew G BlangoAxel A Brakhage
Oct 27, 2018·Folia Microbiologica·Lucélia SantiWalter O Beys-da-Silva
Jun 7, 2019·Diseases of Aquatic Organisms·Juliette DoumayrouAndrew R Wargo
Oct 24, 2020·Tropical Medicine and Infectious Disease·Elerson Matos RochaJayme A Souza-Neto
Sep 13, 2019·Microbial Pathogenesis·J Alberto Patiño-MedinaVictor Meza-Carmen

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Methods Mentioned

BETA
genetic modification
PCR

Software Mentioned

R
SPSS

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