Apr 24, 2020

Genome-wide study of the effect of blood collection tubes on the cell-free DNA methylome

BioRxiv : the Preprint Server for Biology
R. Van PaemelKatleen De Preter

Abstract

Background: The methylation pattern of cfDNA, isolated from liquid biopsies, is gaining substantial interest for diagnosis and monitoring of diseases. We have evaluated the impact of type of blood collection tube and time delay between blood draw and plasma preparation on bisulfite-based cfDNA methylation profiling. Methods: 15 tubes of blood were drawn from three healthy volunteer subjects (BD Vacutainer K2E EDTA spray tubes, Streck Cell-Free DNA BCT tubes, PAXgene Blood ccfDNA tubes, Roche Cell-Free DNA Collection tubes and Biomatrica LBgard blood tubes in triplicate). Samples were either immediately processed or stored at room temperature for 24 or 72 hours before plasma preparation. DNA fragment size was evaluated by capillary electrophoresis. Reduced representation bisulfite sequencing was performed on the cell-free DNA isolated from these plasma samples. We evaluated the impact of blood tube and time delay on several quality control metrics. Results: All preservation tubes performed similar on the quality metrics that were evaluated. Furthermore, a considerable increase in cfDNA concentration and the fraction of it derived from NK cells was observed after a 72-hour time delay in EDTA tubes. Conclusion: The methylation pat...Continue Reading

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Mentioned in this Paper

Microorganism
Health Center
In Vivo
Paracoccidioides brasiliensis
Unicellular Trichome Branch
Pulmonary Fibrosis/Granuloma
Mice, Inbred BALB C
Lung
Granulomatous Disorder
Cessation of Life

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