Experimental hepatitis A virus (HAV) infection in Callithrix jacchus: early detection of HAV antigen and viral fate

Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie
M A PintoA M Gaspar

Abstract

Common marmosets (Callithrixjacchus) were orally inoculated with a Brazilian strain (HAF-203) of hepatitis A virus (HAy). Three monkeys were euthanized at postinoculation hours 6, 12 and 24 to investigate the early events of HAV infection. Following others three inoculated and one control marmosets remained throughout the 46 day to evaluation of viral excretion. Different samples were collected to detect sequential presence of HAV RNA by nested reverse transcription-polymerase chain reaction (RT-PCR) in liver, saliva, bile and stools at 6 hours to 461h days postinoculation. Liver tissues were examined by immunofluorescence assay in a confocal laser-scanning microscope for the presence of HAV antigen. HAV RNA was detected in saliva during the course of the study, in bile from 24 hours to 46 days. in stools from 7 to 46 days and liver at 12 hours postinfection. In immunofluorescence of liver stained preparations, viral antigen was present at six hours after inoculation throughout the remainder of the 46-day study. The animals developed histological and biochemical acute hepatitis after second week postinoculation. Spleen, duodenum, and mesenteric lymph nodes specimens were negative for HAV antigens. This study supports the possib...Continue Reading

References

Apr 5, 1975·Lancet·J L DienstagR H Purcell
Jul 1, 1975·The American Journal of the Medical Sciences·F DeinhardtA W Holmes
Jan 1, 1969·Virchows Archiv. A: Pathology. Pathologische Anatomie·H F Smetana, A D Felsenfeld
Dec 6, 1980·Lancet·M S SmithM Bootsma
Nov 1, 1995·Journal of Medical Virology·L V AsherG D Young
Jul 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H YotsuyanagiS Iino
Apr 25, 2000·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·M A PintoC F Kubelka

❮ Previous
Next ❯

Citations

May 27, 2009·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·V S de PaulaM A Pinto
Jan 19, 2006·Clinical Microbiology Reviews·Omana V NainanHarold S Margolis
Sep 15, 2004·Journal of Clinical Microbiology·Vincent MackiewiczAnne Marie Roque-Afonso
Jun 27, 2008·Journal of Virology·Michelle J SnooksDavid A Anderson
Aug 9, 2012·Seminars in Fetal & Neonatal Medicine·Hideyuki OkanoErika Sasaki
Jan 7, 2014·Development, Growth & Differentiation·Noriyuki KishiHideyuki Okano
Feb 26, 2016·Revista Do Instituto De Medicina Tropical De São Paulo·Walfrido Kühl SvobodaItalmar Teodorico Navarro
Feb 2, 2018·Archives of Virology·Jie-Mei YuZhao-Jun Duan
Mar 1, 2016·Scientific Reports·Jie-mei YuZhao-Jun Duan
Sep 11, 2013·Immunogenetics·Marit K van der WielRonald E Bontrop
Dec 20, 2013·Clinical Liver Disease·Charles Phan, F Blaine Hollinger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.