Experimental infection of germ-free mice with hyper-toxigenic enterohaemorrhagic Escherichia coli O157:H7, strain 6

Journal of Medical Microbiology
Haruhiko TaguchiShigeru Kamiya

Abstract

A mouse enterohaemorrhagic Escherichia coli (EHEC) infection model was developed with a combination of germ-free (GF) mice and hyper-toxigenic EHEC (HT-EHEC) O157:H7 strain 6. The HT-EHEC strain 6 produced both Shiga-like toxin (SLT)-1 1.0 microg/mI and SLT-2 8.2 microg/ml in vitro. Eight-week-old germ-free mice were inoculated intragastrically with 5.0 x 10(7) cfu of HT-EHEC strain 6. All GF mice challenged with the HT-EHEC developed ruffled fur and convulsion of limbs or hindleg weakness within 3 days after the challenge, culminating in death within 5 days. The HT-EHEC colonised well at a level of 10(8) - 10(9) cfu/g of faeces 5 days after the challenge. Both SLT-1 and SLT-2 were demonstrated in the faeces of the mice for 5 days after challenge. Histological examination of the colons of the infected mice showed congestion of the lamina propria mucosa, mild inflammatory cell infiltration and goblet cell depletion. In proximal tubules of the renal cortex, epithelial swelling with scattered necrotic cells was recognised. Endothelial swelling and mononuclear cell infiltration were also observed in the glomeruli. The brain showed acute neuronal necrosis in the cerebrum and slight loss of Purkinje cells in the cerebellum. Passive i...Continue Reading

Citations

Sep 29, 2009·International Journal of Medical Microbiology : IJMM·Julia-Stefanie FrickIngo B Autenrieth
Oct 22, 2005·International Journal of Medical Microbiology : IJMM·Stuart W NaylorJ Christopher Low
Jan 29, 2011·Journal of Biomedicine & Biotechnology·Krystle L Mohawk, Alison D O'Brien
Aug 14, 2010·Gastroenterology Research and Practice·Gakuhei SonIan N Hines
Jun 16, 2004·FEMS Immunology and Medical Microbiology·Motomichi TakahashiShigeru Kamiya
Apr 24, 2009·FEMS Immunology and Medical Microbiology·Shigemitsu TanakaJiro Nakayama
Sep 28, 2017·Infection and Immunity·Bruna C LustriCristiano G Moreira
Jan 16, 2019·Current Protocols in Mouse Biology·Máximo E LangeG Douglas Inglis
Sep 3, 2010·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Zivile D BékássyDiana Karpman
Oct 9, 2012·The Journal of Clinical Investigation·Emily M MallickDavid B Schauer
Aug 4, 2006·Applied and Environmental Microbiology·Haiqing ShengCarolyn J Hovde
Apr 30, 2008·Infection and Immunity·Kathryn A EatonThomas S Whittam
Nov 8, 2014·Veterinary Pathology·C ErsdalK-I Lie
Feb 9, 2017·Veterinary Pathology·K A EatonC J Alteri
Jan 24, 2003·Infection and Immunity·Yasuyuki ImaiMasaki Miyake
Oct 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julia GeiselJulia-Stefanie Frick
Nov 28, 2013·Complementary Therapies in Medicine·Michal MegoVladimir Zajac
Feb 26, 2021·Frontiers in Molecular Biosciences·Meng Zhou, Jiang Zhao
Dec 23, 2014·Microbiology Spectrum·Fumiko Obata, Tom Obrig
Jun 25, 2015·Microbiology Spectrum·Jennifer M Ritchie

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