Experimental infection of the honeybee (Apis mellifera L.) with the chronic bee paralysis virus (CBPV): infectivity of naked CBPV RNAs

Virus Research
Aurore ChevinMagali Ribière

Abstract

Chronic paralysis is an infectious and contagious disease of the honeybee (Apis mellifera L.) and is caused by the chronic bee paralysis virus (CBPV). This disease leads to death in adult bees and is therefore a serious threat for colony health. CBPV is a positive single-stranded RNA virus and its genome is composed of two RNA segments, RNA 1 and RNA 2, 3674 nt and 2305 nt, respectively. Although CBPV shares some characteristics with viruses classified into families Nodaviridae and Tombusviridae, it has not been assigned to any viral taxa yet. The characterisation of CBPV proteins and their functions are needed to better understand the mechanisms of CBPV infection. However, since honeybee cell lines are not yet available, experimental infection of adult bees is the only method currently available to propagate the virus. With the objective of studying CBPV proteins using the viral genome, we used experimental infection in adult bees to evaluate the infectivity of naked CBPV RNAs by direct inoculation. Our results demonstrated that an injection of naked RNAs, ranging from 10(9) to 10(10) CBPV copies, caused chronic paralysis. Bees inoculated with naked RNA showed chronic paralysis signs 5 days after inoculation. Moreover, injecte...Continue Reading

References

Jun 1, 1965·Journal of Invertebrate Pathology·L Bailey
Aug 1, 1967·The Annals of Applied Biology·L Bailey
May 22, 2002·Journal of Virology·Pei-Yong ShiKristen A Bernard
Dec 6, 2002·The Journal of General Virology·Mongi BenjeddouSean Davison
Jan 1, 2003·Journal of Invertebrate Pathology·Mongi BenjeddouSean Davison
Nov 1, 1963·Virology·L BAILEYR D WOODS
Nov 22, 2005·The Journal of General Virology·Constanze Yue, Elke Genersch
Dec 8, 2006·Journal of Virology·Mark Boyce, Polly Roy
Sep 8, 2007·Science·Diana L Cox-FosterW Ian Lipkin
Nov 17, 2009·Journal of Invertebrate Pathology·Magali RibièrePhilippe Blanchard
Dec 20, 2011·Applied and Environmental Microbiology·Benjamin DainatPeter Neumann

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Citations

Mar 8, 2020·International Journal of Molecular Sciences·Sa YangChunsheng Hou

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