Experimental infections of Pacific oyster Crassostrea gigas using the Australian ostreid herpesvirus-1 (OsHV-1) µVar strain

Diseases of Aquatic Organisms
Ika Paul-PontRichard Whittington

Abstract

In Australia, the spread of the ostreid herpesvirus-1 microvariant (OsHV-1 µVar) threatens the Pacific oyster industry. There is an urgent need to develop an experimental infection model in order to study the pathogenesis of the virus under controlled laboratory conditions. The present study constitutes the first attempt to use archived frozen oysters as a source of inoculum, based on the Australian OsHV-1 µVar strain. Experiments were conducted to test (1) virus infectivity, (2) the dose-response relationship for OsHV-1, and (3) the best conditions in which to store infective viral inoculum. Intramuscular injection of a viral inoculum consistently led to an onset of mortality 48 h post-injection and a final cumulative mortality exceeding 90%, in association with high viral loads (1 × 105 to 3 × 107 copies of virus mg-1) in dead individuals. For the first time, an infective inoculum was produced from frozen oysters (tissues stored at -80°C for 6 mo). Storage of purified viral inoculum at +4°C for 3 mo provided similar results to use of fresh inoculum, whereas storage at -20°C, -80°C and room temperature was detrimental to infectivity. A dose-response relationship for OsHV-1 was identified but further research is recommended to ...Continue Reading

References

Dec 18, 2004·The Journal of General Virology·Andrew J DavisonTristan Renault
Mar 18, 2008·Journal of Virological Methods·J F PepinT Renault
Sep 22, 2011·Microbial Ecology·Colleen A Burge, Carolyn S Friedman
Apr 1, 2014·Journal of Invertebrate Pathology·Amélie SegarraTristan Renault

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Citations

Feb 18, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Fabrice PernetRichard J Whittington
Mar 17, 2018·Viruses·Timothy J Green, Peter Speck
Mar 7, 2020·Diseases of Aquatic Organisms·Olivia LiuRichard J Whittington
Feb 18, 2021·Journal of Invertebrate Pathology·Maria Prado-AlvarezCamino Gestal
Aug 28, 2020·The Science of the Total Environment·Carolyn S FriedmanColleen A Burge

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