Experimental investigation of radiobiology in head and neck cancer cell lines as a function of HPV status, by MTT assay

Scientific Reports
Paul ReidEva Bezak

Abstract

Head and neck cancers (HNCs) are aggressive epithelial tumours frequently treated using radiation. HNC biology shows distinctions dependent on the oncologic involvement of the human papilloma virus (HPV). Clinically, HPV positive HNCs respond better to radiotherapy but few in vitro data demonstrate radiobiological differences explaining differences in clinical outcomes. This pilot study examined radiobiological responses to irradiation and subsequent regeneration in two HNC cell lines (HPV positive and negative). A novel approach was taken to develop generational cultures of HNC cell lines, UM-SCC-1 (HPV negative) and UM-SCC-47 (HPV positive). MTT assays were used to determine surviving metabolic activity as a function of dose following 6 MV X-ray irradiation. Parallel cultures surviving 4 Gy irradiation (not analysed) were re-cultured and passaged to develop subsequent generations which were re-irradiated and analysed for generational change in radiation response. Second and 3rd generations of UM-SCC-1 showed decreasing metabolic activity with dose but little difference was evident in surviving fractions between these generations. Significantly lower metabolic activity in the 3rd generation at <6 Gy, compared to the 2nd genera...Continue Reading

References

Jan 1, 1988·Acta Oncologica·H R WithersB Maciejewski
Jan 1, 1995·International Journal of Technology Assessment in Health Care
Jan 1, 1994·International Journal of Radiation Biology·M C Joiner
Jun 16, 2001·ORL; Journal for Oto-rhino-laryngology and Its Related Specialties·A FerlitoV Mondin
Oct 12, 2002·BMJ : British Medical Journal·R J Sanderson, J A D Ironside
Nov 6, 2003·Gynecologic Oncology·U MatulonisUNKNOWN Dana Farber-Harvard Cancer Care
Mar 18, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Pernille LassenJens Overgaard
Sep 18, 2009·Head & Neck·J Chad BrennerThomas E Carey
Nov 10, 2009·International Journal of Radiation Biology·Apostolos MenegakisMechthild Krause
Jul 6, 2010·Molecular Oncology·Sean P McDermott, Max S Wicha
Dec 17, 2010·Nature Reviews. Cancer·C René LeemansRuud H Brakenhoff
Mar 8, 2013·Radiation Research·Stéphanie BlockhuysCarlos De Wagter
Apr 23, 2013·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Thorsten RieckmannMalte Kriegs
Jun 12, 2013·Cancer Research·Randall J KimplePaul M Harari
Nov 20, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Anil K ChaturvediMaura L Gillison
Apr 10, 2014·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Andrea ArenzClaus Wittekindt

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Citations

Oct 22, 2019·BioFactors·Kiruthika SundarrajEkambaram Perumal
Apr 24, 2020·Frontiers in Cellular and Infection Microbiology·Simona CitroSusanna Chiocca

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Methods Mentioned

BETA
surgical resection
X-ray
biopsies

Software Mentioned

GraphPad
Prism

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