Experimental Mapping of the Canine KCNJ2 and KCNJ12 Gene Structures and Functional Analysis of the Canine K(IR)2.2 ion Channel.

Frontiers in Physiology
Marien J C HoutmanMarcel A G van der Heyden

Abstract

For many model organisms traditionally in use for cardiac electrophysiological studies, characterization of ion channel genes is lacking. We focused here on two genes encoding the inward rectifier current, KCNJ2 and KCNJ12, in the dog heart. A combination of RT-PCR, 5'-RACE, and 3'-RACE demonstrated the status of KCNJ2 as a two exon gene. The complete open reading frame (ORF) was located on the second exon. One transcription initiation site was mapped. Four differential transcription termination sites were found downstream of two consensus polyadenylation signals. The canine KCNJ12 gene was found to consist of three exons, with its ORF located on the third exon. One transcription initiation and one termination site were found. No alternative splicing was observed in right ventricle or brain cortex. The gene structure of canine KCNJ2 and KCNJ12 was conserved amongst other vertebrates, while current GenBank gene annotation was determined as incomplete. In silico translation of KCN12 revealed a non-conserved glycine rich stretch located near the carboxy-terminus of the K(IR)2.2 protein. However, no differences were observed when comparing dog with human K(IR)2.2 protein upon ectopic expression in COS-7 or HEK293 cells with respect...Continue Reading

Citations

Nov 30, 2012·Pflügers Archiv : European journal of physiology·Rosanne VarkevisserMarcel A G van der Heyden
Nov 26, 2015·European Journal of Pharmacology·Marcel A G van der Heyden, Thomas Jespersen
Jul 22, 2014·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Yuhong WangJose F Huizar
Dec 3, 2016·The Journal of General Physiology·Daniel R MirandaAndrew A Voss
Jul 25, 2019·Frontiers in Physiology·Muge QileMarcel A G van der Heyden

❮ Previous
Next ❯

Datasets Mentioned

BETA
HM209045
HM209046
BK007085
HQ378597

Methods Mentioned

BETA
PCR
transfection

Software Mentioned

pClamp
Microcal
Clustal
Microcal Origin
Vector NTI suite

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

© 2022 Meta ULC. All rights reserved