Experimental methods for kinetic study of suicide substrates

Journal of Enzyme Inhibition
F Garcia-CanovasA M Vazquez

Abstract

The kinetic study of the enzymatic inactivation originated by suicide substrates can be carried out by means of two alternative approaches. One method considers the substrate concentration as practically constant during the assay time and provides explicit equations of product concentration vs. time. The other method involves the significant consumption of the substrate, yielding implicit equations of time vs. product concentration. The utility of both methods is discussed and adequate experimental conditions for their correct application are established.

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Citations

May 19, 2000·The Biochemical Journal·A N HinerM Acosta
Feb 15, 2001·The Biochemical Journal·J Hernández-RuizM Acosta
Jul 8, 2009·International Journal of Molecular Sciences·Te-Sheng Chang
Apr 13, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Jose Luis Muñoz-MuñozFrancisco García-Cánovas
Mar 11, 2010·Biochimica Et Biophysica Acta·J L Muñoz-MuñozJ N Rodríguez-López
Mar 7, 2013·Chemical Biology & Drug Design·Jinbing LiuLiangzhong Zhao
Jun 17, 2010·IUBMB Life·Jose Luis Muñoz-MuñozJose Neptuno Rodríguez-López
Nov 18, 2008·Biochimica Et Biophysica Acta·Jose Luis Munoz-MunozJose Neptuno Rodriguez-Lopez
May 25, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Howard J BurtAleksandra Galetin
Feb 2, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·M KhosranehA A Moosavi-Movahedi
Jul 24, 2008·The Biochemical Journal·Jose L Muñoz-MuñozJose N Rodríguez-López
Oct 30, 1998·Bio Systems·R VarónB H Havsteen
Apr 13, 1994·Biochimica Et Biophysica Acta·M García MorenoF García Cánovas

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