Experimental methods in aquatic respirometry: the importance of mixing devices and accounting for background respiration

Journal of Fish Biology
G G RodgersTimothy D Clark

Abstract

In light of an increasing trend in fish biology towards using static respirometry techniques without the inclusion of a mixing mechanism and without accurately accounting for the influence of microbial (background) respiration, this paper quantifies the effect of these approaches on the oxygen consumption rates (ṀO2 ) measured from juvenile barramundi Lates calcarifer (mean ± s.e. mass = 20·31 ± 0·81 g) and adult spiny chromis damselfish Acanthochromis polyacanthus (22·03 ± 2·53 g). Background respiration changed consistently and in a sigmoidal manner over time in the treatment with a mixing device (inline recirculation pump), whereas attempts to measure background respiration in the non-mixed treatment yielded highly variable estimates of ṀO2 that were probably artefacts due to the lack of water movement over the oxygen sensor during measurement periods. This had clear consequences when accounting for background respiration in the calculations of fish ṀO2 . Exclusion of a mixing device caused a significantly lower estimate of ṀO2 in both species and reduced the capacity to detect differences between individuals as well as differences within an individual over time. There was evidence to suggest that the magnitude of these effe...Continue Reading

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Citations

Nov 26, 2015·Journal of Fish Biology·M B S SvendsenJ F Steffensen
Oct 26, 2016·Journal of Fish Biology·S S KillenL G Halsey
Sep 23, 2016·The Journal of Experimental Biology·Lauren E NadlerMark I McCormick
May 6, 2019·The Journal of Experimental Biology·Yangfan ZhangAnthony P Farrell
Jul 28, 2020·Journal of Fish Biology·Edurne BlancoMarta Moyano
Jul 21, 2017·The Journal of Experimental Biology·Michael J LouisonCory D Suski
Sep 27, 2018·Conservation Physiology·Ian A BouyoucosJodie L Rummer
Nov 7, 2019·Conservation Physiology·Bryan D FrenetteKeith B Gido
Dec 10, 2016·Conservation Physiology·Lauren E NadlerPhilip L Munday
Jul 28, 2017·Scientific Reports·N A HerbertA Kunzmann
Feb 13, 2019·Conservation Physiology·Sergey MorozovJuha Merilä
Aug 14, 2019·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Warren W BurggrenJosé Fernando Méndez-Sánchez
Sep 15, 2021·The Journal of Experimental Biology·Shaun S KillenDavid J McKenzie
Oct 15, 2021·The Journal of Experimental Biology·Emily A HardisonErika J Eliason

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