Experimental models for cardiac regeneration

Nature Clinical Practice. Cardiovascular Medicine
Ana SánchezJavier García-Sancho

Abstract

Simple ex vivo or in vitro models are most useful for testing putative cell therapy protocols, as they allow quick and controlled screening of variants and possible improvements. We discuss here three different models: coculture of precursors of human bone marrow cells (BMCs) with mouse heart slices bearing a cryogenic lesion; coculture of human BMCs and rat cardiomyocytes separated by a porous membrane that allows passage of soluble substances but prevents migration of nuclear material; and injection of human BMCs in developing chick heart bearing burn lesions. Our results indicate that the damaged areas express specific genes such as MPC1 and SDF1, and that some human BMCs migrate and graft near the lesion, where they can originate cells with a cardiac phenotype that produce human cardiac proteins. The frequency of this transformation is, however, very low. Understanding the factors that determine and regulate nuclear reprogramming and transdifferentiation would be crucial to appraising the contribution of these phenomena to cardiac regeneration and, eventually, to modulating them with therapeutic intent.

References

Apr 5, 2001·Nature·D OrlicP Anversa
Jan 24, 2002·Nature·Piero Anversa, Bernardo Nadal-Ginard
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Jan 9, 2003·Lancet·Christof StammGustav Steinhoff
Jul 25, 2003·The Journal of Thoracic and Cardiovascular Surgery·Takayuki SaitoRay C-J Chiu
Aug 2, 2003·Blood·Erica L HerzogDiane S Krause
Sep 11, 2004·Circulation Research·Francisco Fernández-AvilésAna Sánchez
Jan 8, 2005·Circulation Research·Florian P Limbourg, Helmut Drexler
Feb 24, 2005·Stem Cells and Development·Claudia LangeAxel R Zander

❮ Previous
Next ❯

Citations

Apr 9, 2008·Stem Cells and Development·Nuria Torre-PérezJuan M Hurlé
Jul 14, 2011·Regenerative Medicine·Yongsung HwangShyni Varghese
Jun 19, 2010·Journal of Cardiovascular Translational Research·Ana ArmiñánPilar Sepúlveda
Apr 14, 2007·Cell Death and Differentiation·A Sánchez, J García-Sancho

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.