PMID: 3745274Sep 1, 1986Paper

Experimental modification of PC12 neurite shape with the microtubule-depolymerizing drug Nocodazole: a serial electron microscopic study of neurite shape control

The Journal of Cell Biology
J R Jacobs, J K Stevens

Abstract

The microtubule-depolymerizing drug Nocodazole has been used to experimentally manipulate the form of PC12 neurites. Both time-lapse photography and serial electron microscopy demonstrate that microtubule depolymerization leads to varicosity formation due to a clustering of membranous organelles in young neurites (nerve growth factor activated within 7 d). Neurites that have been nerve growth factor activated 7 or more d before Nocodazole application are resistant to microtubule depolymerization. These data and data from previous papers has been combined in an attempt to predict quantitatively the volume and the shape of a neurite. The relationship is described mathematically by Vn = 4.52 Vo + 0.0054 MTl, where Vn is local neurite volume, Vo is organelle volume, and MTl is MT length (the constant, 0.0054 is micron2), and 4.52 is the obligatory volume constant derived from serial electron microscopic studies. The equation predicts the total volume of neurites despite alterations of morphology due to Nocodazole and despite changes in morphology during development.

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Citations

Dec 1, 1990·Journal of Neurocytology·M M GreenbergJ K Stevens
Dec 1, 1987·Journal of Neurocytology·L A WhiteS R Heidemann
Feb 1, 1990·Brain Research. Developmental Brain Research·T B SheaR A Nixon
Mar 27, 2004·European Journal of Pharmacology·Matsumi YamazakiHiroshi Hatanaka
Aug 1, 1988·The Journal of Cell Biology·T J DennerllS R Heidemann
Sep 1, 1990·Journal of Neurochemistry·T Sarafian, M A Verity
Nov 5, 2003·Journal of Neurobiology·Leif Dehmelt, Shelley Halpain
Feb 18, 2010·Biophysical Journal·Roberto BernalPramod A Pullarkat
Mar 1, 1989·Experimental Cell Research·L SerranoJ Avila
Jan 1, 1987·Cell Motility and the Cytoskeleton·J R Jacobs, J K Stevens
Jul 28, 2018·Frontiers in Cellular Neuroscience·Fernanda GárateRoberto Bernal
Dec 31, 2015·Physical Biology·Fernanda GárateRoberto Bernal
Mar 8, 1989·The Journal of Comparative Neurology·J S Deitch, E W Rubel

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