Abstract
Male CBA/J mice, sensitized by cutaneous inoculation with viable Candida albicans blastospores, were used to study in vivo and in vitro cellular immune responses. Three antigens of C. albicans, viz., a cell wall glycoprotein (GP), a membrane extract (ppt-HEX), and soluble cytoplasmic substances (SCS), were used in vitro in a lymphocyte stimulation assay, whereas the GP and ppt-HEX were used in vivo to detect delayed hypersensitivity by the footpad assay. Delayed hypersensitivity to GP and ppt-HEX was transferred from sensitized donors to naive recipients with peritoneal exudate cells and not with serum. Moreover, the transfer of the reactivity to ppt-HEX was abrogated by the prior treatment of the transfer suspension with anti-theta 1.2 serum and complement. The in vitro lymphocyte response to GP and ppt-HEX correlated qualitatively with the in vivo responses. SCS, a preparation shown to be ineffective in vivo previously, did stimulate lymphocytes from sensitized animals in vitro. The in vitro response to Candida antigens, as well as phytohemagglutinin, was abolished by treatment of the lymphocyte suspension with anti-thymocyte 1.2 serum before assay, whereas anti-immunoglobulin serum had less effect on these responses. The in ...Continue Reading
References
May 1, 1976·The Journal of Infectious Diseases·D RifkindM Dinowitz
Oct 1, 1977·American Journal of Clinical Pathology·A C FergusonE R Stiehm
Feb 1, 1978·Infection and Immunity·D K GigerJ T McQuitty
Jun 1, 1977·Irish Journal of Medical Science·A M HassettG M Mullins
Jul 1, 1978·Clinical Immunology and Immunopathology·T J Rogers, E Balish
Apr 1, 1978·Infection and Immunity·T J Rogers, E Balish
Apr 1, 1978·Infection and Immunity·J E Domer, S A Moser
Mar 1, 1978·Mykosen·I Helander
May 1, 1976·The Journal of Infectious Diseases·D RifkindM Dinowitz
Mar 1, 1976·Infection and Immunity·J E Domer
Jan 1, 1977·Journal of Immunological Methods·E BrummerH S Lawrence
Jan 11, 1976·The Journal of Clinical Investigation·R W SteeleL O Gentry
Jan 1, 1976·The Journal of Infectious Diseases·D RifkindM Dinowitz
Sep 1, 1974·Infection and Immunity·R J North, G Spitalny
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Dec 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·R H Alford, R M Des Prez
Jan 1, 1974·International Archives of Allergy and Applied Immunology·D E BiceJ Salvaggio
Jul 1, 1970·Transplantation·A Cohen, M Schlesinger
Mar 20, 1969·The New England Journal of Medicine·R CohenM H Kalser
Sep 29, 1966·The New England Journal of Medicine·D C ShannonK F Austen
Apr 1, 1960·The Biochemical Journal·E D KORN, D H NORTHCOTE
Citations
Nov 25, 1983·Mycopathologia·R LevyL Barr-Nea
Jan 1, 1989·Critical Reviews in Microbiology·J E Domer
Nov 1, 1992·Scandinavian Journal of Immunology·J W van 't WoutR van Furth
Jul 1, 1995·Clinical Microbiology Reviews·D K Wagner, P G Sohnle
Nov 1, 1989·Infection and Immunity·E CenciF Bistoni
Mar 1, 1989·Infection and Immunity·J E DomerR N Befidi-Mengue
May 1, 1987·Antimicrobial Agents and Chemotherapy·J E Domer, R F Hector
Jul 1, 1985·Infection and Immunity·E W Carrow, J E Domer
Dec 1, 1981·Infection and Immunity·R F HectorJ E Domer
Feb 1, 1986·Infection and Immunity·F BistoniA Cassone
May 1, 1992·Infection and Immunity·R E GarnerJ E Domer
Dec 1, 1984·Clinical Immunology and Immunopathology·E W CarrowJ E Domer
Aug 1, 1990·Infection and Immunity·R E GarnerJ E Domer