PMID: 1194930Dec 1, 1975Paper

Experimental necrosis and arrest of proliferation of Schwann cells by cytosine arabinoside

Journal of Neurocytology
A J AguayoG M Bray

Abstract

In developing rat cervical sympathetic trunks, Schwann cells proliferate intensely during the first week after birth but axonal populations do not increase. Thus, experimental inhibition of DNA synthesis should affect Schwann cells and spare axons whose cell bodies are not dividing. The present investigation was aimed at determining the effects of an inhibitor of DNA synthesis-cytosine arabinoside (ara-C)--on axons and Schwann cells in developing nerves. Ara-C (60 mg kg(-1) body weight) was injected subcutaneously to newborn rats every six hours for 36 hours. At intervals from 2--4 days of age, animals were given tritiated thymidine (4 muCi per gram body weight) to label Schwann cells in synthesis phase. One hour later rats were killed by systemic perfusion of phosphate buffered glutaraldehyde. Cervical sympathetic trunks (CST's) and sciatic nerves were removed and processed for radioautography and electron microscopy (EM). Labelled and unlabelled Schwann cell nuclei were counted to determine the labelling index (LI%) for each nerve. By the end of ara-C treatment there was almost complete absence of labelling but LI's rose sharply 24 hours after discontinuing ara-C. By EM axons appeared normal; Schwann cells, however, showed pr...Continue Reading

References

Jan 1, 1975·Journal of the Neurological Sciences·J K Korthals, H M Wiśniewski
Nov 1, 1975·Journal of Neuropathology and Experimental Neurology·G M Bray, A J Aguayo
Mar 1, 1976·Journal of Neuropathology and Experimental Neurology·A J AguayoE Sweezey
Feb 1, 1973·Journal of the Neurological Sciences·W G Bradley, M Jenkison
Oct 1, 1965·Archives of Neurology·R F Mayer
Jan 1, 1969·Virchows Archiv. A: Pathology. Pathologische Anatomie·F CarlsenH Schmalbruch
Oct 1, 1971·The Journal of Pathology·I TorresC E Oxnard
Jan 1, 1972·Acta Neuropathologica·A J AguayoG M Bray
Oct 29, 1968·Biochimica Et Biophysica Acta·C Atkinson, K A Stacey
Nov 30, 1971·Annals of the New York Academy of Sciences·S S Cohen
Aug 1, 1972·Archives of Neurology·W A Farris, M E Blaw
Oct 1, 1971·The Journal of Clinical Investigation·B C LampkinA M Mauer
Jan 1, 1959·Quarterly Journal of Experimental Physiology and Cognate Medical Sciences·A PETERS, A R MUIR

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Citations

Jun 14, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Joseph M BreierTimothy J Shafer
May 1, 1978·Neuropathology and Applied Neurobiology·S M Hall
Jan 1, 1979·Annals of the New York Academy of Sciences·A J AguayoS C Perkins
Jul 29, 2004·Clinical and Laboratory Haematology·W L OsborneA N Parker
Jul 11, 2006·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Takeshi SaitoNoriko Usui
Dec 30, 2014·Environmental Toxicology and Pharmacology·Eui-Ju HongEui-Bae Jeung
Feb 1, 1981·The American Journal of Anatomy·M E Todd, M K Tokito

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