Experimental repair of segmental bone defects in rabbits by angiopoietin-1 gene transfected MSCs seeded on porous β-TCP scaffolds

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Le CaoBingfang Zeng

Abstract

Segmental bone defect repair remains a clinical and experimental challenge in tissue engineering with increasing focus on angiogenesis in the bone substitutes. The objective of this study was to investigate the osteogenic effects of angiopoietin-1 (Ang-1) gene transfected bone marrow-derived mesenchymal stem cells (MSCs) seeded on porous β-TCP scaffolds. This bone substitute (experimental group) and MSCs/β-TCP compounds (control group) were implanted into 15 mm segmental bone defects of the radii of 30 New Zealand white rabbits, with platelet-rich plasma injected at the same time. Bone regeneration and angiogenesis were assessed by Scanning electron microscope (SEM), X-ray, histology, immunohistology, and biomechanical outcome measurements made on the 2nd, 4th, 8th, and 12th week after the operation. In vitro, the amount of proliferation and differentiation of Ang-1 gene transfected MSCs was found to be gross increased than that of the control groups. In vivo, a significantly increased amount of new bone formation accompanied by active capillary vasculature regeneration was observed in the pores of the scaffolds which had been seeded with Ang-1 gene transfected MSCs, as compared with the control groups. The biomechanical test c...Continue Reading

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Citations

Dec 10, 2015·BioMed Research International·R V DeevA A Isaev
Mar 18, 2015·Nature Reviews. Rheumatology·Benjamin D Smith, Daniel A Grande
Mar 1, 2014·Tissue Engineering. Part a·Concetta FerrettiMonica Mattioli-Belmonte
Dec 6, 2018·Current Stem Cell Research & Therapy·Benjamin MesureÉmilie Velot
May 28, 2015·Drug Delivery and Translational Research·José R García, Andrés J García
Jun 27, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Bethany E LiebigLaurie R Goodrich
Jul 9, 2020·Tissue Engineering. Part B, Reviews·Maximilian M MengerTina Histing

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