PMID: 20632502Jul 17, 2010Paper

Experimental research of antibacterial property and release characteristics of Titania and Ag containing nano-hydroxyapatite/polyamide 66 composite bone filling material in vitro

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Minpeng LuYu-Bao Li

Abstract

Titania and Ag containing nano-hydroxyapatite/polyamide 66 (TiO2-Ag-nHA/PA66) composite bone filling material has good biocompatibility and biological safety. To investigate the antibacterial effect and Ag+ release characteristics of TiO2-Ag-nHA/PA66 composite bone filling material containing different concentrations of Ag+ in vitro. The n-HA/PA66 composite bone filling material A1 (material A1) was prepared by co-polymerization method, and TiO2-Ag-nHA/PA66 composite bone filling materials A2 and A3 (materials A2 and A3) were prepared by the same way containing Ag+ of 0.22wt% and 0.64wt%, respectively, and the TiO2 content was 2.35wt%. The materials A2 and A3 were respectively immersed in 50 mL simulated body fluid (SBF), and Ag+ concentration was measured by atomic absorption spectrometry at 1, 3, 7, 14, 21, and 49 days. The inhibition ring test and colony count method were used to evaluate antibiotic effect against Staphylococcus aureus and Escherichia coli, the anti-adhesion capacity of Staphylococcus aureus and Escherichia coli was observed by scanning electron microscope (SEM). There was no significant difference in the Ag+ concentration between materials A2 and A3 at 1 day and 3 days (P > 0.05); and there were significant...Continue Reading

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