PMID: 6399821Jan 1, 1984Paper

Experimental studies of folding kinetics and structural dynamics of small proteins

Advances in Biophysics
K Kuwajima, F X Schmid

Abstract

Main points given in the above reports can be summarized as follows. Multiple unfolded forms exist for lysozyme as well as for RNase. The existence of fast- and slow-folding forms appears to be a general phenomenon; it has been confirmed for a number of globular proteins, which contain proline residues. The major slow refolding reaction of RNase A is a sequential process via structural intermediates. A rapidly formed intermediate has also been detected on the direct UF----N refolding pathway of lysozyme. The activated state for folding of lysozyme shows a conformation similar to the native protein in terms of packing of hydrophobic groups. This suggests that, in terms of compactness, the rate-limiting step occurs at a late stage of the refolding process. A protein homologous to lysozyme, alpha-lactalbumin, shows similar kinetics although alpha-lactalbumin shows an apparent equilibrium unfolding intermediate. The location of the rate-limiting step close to the native state has also been suggested for other proteins. It still remains open whether this is a general property of protein folding reactions. As shown for the unfolding of Mb, the multi-probe kinetic measurements will be a powerful tool for investigating the mechanism of...Continue Reading

Citations

May 1, 1992·Protein Science : a Publication of the Protein Society·W Wilcox, D Eisenberg
Jul 3, 2013·Journal of Molecular Modeling·Barbara KalinowskaIrena Roterman
Oct 13, 2012·Journal of Pharmaceutical Sciences·Santosh V ThakkarC Russell Middaugh
Sep 21, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Chia-Ching ChangLou-Sing Kan
Mar 27, 2015·Journal of Computer-aided Molecular Design·Barbara KalinowskaIrena Roterman
Jan 23, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aashka Y PatelGregory A Caputo
Aug 25, 2004·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Chia-Ching ChangLou-Sing Kan
Jun 6, 2003·Biochemical and Biophysical Research Communications·Yi-Liang LiuLou-Sing Kan

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