Experimental study of vascularized tissue-engineered bone grafts

Plastic and Reconstructive Surgery
Kenji KawamuraYoshinori Takakura

Abstract

Vascularized bone grafting has become a useful method for treatment of large bone defects and infected nonunions that lack adequate blood supply. This method has disadvantages, however, such as donor-site complications. To overcome these disadvantages, the authors developed a new method for creating vascularized tissue-engineered bone grafts in an experimental model. Fisher rat bone marrow mesenchymal stem cells were cultured for 2 weeks in fully opened, interconnected, porous hydroxyapatite ceramic. The composites of mesenchymal stem cells and hydroxyapatite were implanted in the medial calf region together with the saphenous vascular bundle in syngeneic rats. Two weeks after implantation, the vascular pedicle with the implants was exposed and covered with silicone rubber to prevent vascular invasion through surrounding tissues (vascularized mesenchymal stem cell/hydroxyapatite implants). In addition, nonvascularized mesenchymal stem cell/hydroxyapatite implants were created with a ligated vascular pedicle. Implants of vascularized hydroxyapatite alone were prepared as a control. All implants were retrieved 4 weeks after surgery. Histologically, de novo bone formations were observed in the vascularized implants. This was in co...Continue Reading

References

May 1, 1975·Plastic and Reconstructive Surgery·G I TaylorF J Ham
Jan 1, 1989·Progress in Growth Factor Research·J M Wozney
Jan 1, 1989·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H OhgushiA I Caplan
Jun 1, 1986·Plastic and Reconstructive Surgery·S R ColenJ G McCarthy
Jan 1, 1988·Journal of Cell Science. Supplement·M Owen
Dec 1, 1986·Calcified Tissue International·K H Wlodarski, A H Reddi
Nov 1, 1988·Cell and Tissue Research·C ManiatopoulosA H Melcher
Dec 1, 1985·Plastic and Reconstructive Surgery·H NettelbladA J Weiland
May 1, 1995·Journal of Reconstructive Microsurgery·H YajimaO Kisanuki
Nov 1, 1993·Journal of Reconstructive Microsurgery·S MizumotoA J Weiland
Mar 1, 1996·Plastic and Reconstructive Surgery·H IshidaY Dohi
Nov 1, 1996·Journal of Biomedical Materials Research·H OhgushiY Suwa
Nov 11, 1999·Journal of Biomedical Materials Research·H Ohgushi, A I Caplan
Dec 18, 2001·Journal of Biomedical Materials Research·Noriyuki TamaiHideki Yoshikawa
Jan 31, 2002·Plastic and Reconstructive Surgery·Arun K GosainJohn L Ricci
Aug 22, 2002·The Journal of Bone and Joint Surgery. British Volume·S Govender, K P S Kumar

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Citations

May 24, 2007·Tissue Engineering·Hossein HosseinkhaniYasuhiko Tabata
Jan 9, 2010·Plastic and Reconstructive Surgery·Susumu Tamai
Sep 25, 2009·The Tohoku Journal of Experimental Medicine·Zhenyu ZhaoZhenxing Si
Jun 6, 2014·BioMed Research International·Junjun FanGuoxian Pei
Mar 1, 2012·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Zhiwei DongYanpu Liu
Apr 10, 2007·Clinics in Plastic Surgery·Maria Siemionow, Galip Agaoglu
Sep 15, 2011·Journal of Tissue Engineering and Regenerative Medicine·Chien-Yuan WangShih-Chieh Hung
Dec 17, 2009·Annals of Plastic Surgery·Takahisa OkudaHiroshi Mizuno
Apr 28, 2016·Plastic and Reconstructive Surgery·Kenichi NakanoYasuhito Tanaka
Oct 2, 2019·Bioengineering & Translational Medicine·Lauren A HerronHasan E Abaci
Dec 4, 2016·Tissue Engineering. Part B, Reviews·Johnathan NgGordana Vunjak-Novakovic
Jul 27, 2018·Frontiers of Medicine·Rui ShiWei Tian
Aug 20, 2019·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Akira MurayamaKatsushi Takeshita

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