Experimental study on differences in clivus chordoma bone invasion: an iTRAQ-based quantitative proteomic analysis

PloS One
Zhen WuChunjiang Yu

Abstract

Although a bone tumor, significant differences in the extent of bone invasion exist in skull base chordoma, which directly affect the extent of surgical resection, and have an impact on its prognosis. However, the underlying mechanism of the phenomenon is not clearly understood. Therefore, we used an iTRAQ-based quantitative proteomics strategy to identify potential molecular signatures, and to find predictive markers of discrepancy in bone invasion of clivus chordoma. According to bone invasive classification criteria, 35 specimens of clivus chordoma were calssified to be either endophytic type (Type I) or exophytic type (Type II). An initial screening of six specimens of endophytic type and six of exophytic was performed, and 250 differentially expressed proteins were identified. Through the GO and IPA analysis, we found evidence that the expression of inflammatory activity-associated proteins up-regulated in endophytic type, whereas the expression of cell motility-associated proteins up-regulated in exophytic ones. Moreover, TGFβ1 and mTOR signal pathway seemed to be related with bone invasion. Thus, TGFβ1, PI3K, Akt, mTOR, and PTEN were validated in the following 23 samples by immune histochemistry and Western blot. The exp...Continue Reading

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Citations

Jan 27, 2016·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Ke WangJunting Zhang
Jul 8, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Haibo ZhuYazhuo Zhang
Jan 12, 2020·World Neurosurgery·Ke WangZhen Wu
Sep 25, 2018·The American Journal of Pathology·Roberto J DiazJames T Rutka
Oct 23, 2020·Clinical Neurology and Neurosurgery·Klaus ZweckbergerAndreas Unterberg

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Methods Mentioned

BETA
Protein Assay
surgical resection

Software Mentioned

Panther
Ingenuity Pathway Analysis ( IPA )
Mascot
IPA
Scaffold
SPSS11

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