Experimental survey of non-clonogenic viability assays for adherent cells in vitro

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Ingrida MickuvieneB Juodka

Abstract

Results of rapid cell viability assays were experimentally compared in order to reveal the most suitable test for in vitro investigations of the combination of photodynamic therapy (PDT) with chemotherapeutic drugs. meso-Tetra(3-hydroxyphenyl)-chlorin (m-THPC) accumulating in cell membranes and meso-tetra(4-sulfonatophenyl)-porphin (TPPS4) accumulating in lysosomes were used as photosensitisers. Doxorubicin that localises, mainly, to nucleus and vincristine that binds to microtubules were used as cytostatic drugs. Two adherent rodent cell lines, baby hamster kidney (BHK-21) and murine hepatoma (MH-22A), were used to examine the contribution of a cell. We tested cytotoxicity assays of the main groups of fast (non-clonogenic) methods of cell viability measuring. Plasma membrane integrity was estimated by trypan blue exclusion and LDH leakage, metabolic activity was tested by [3H]-thymidine incorporation and MTT assay, loss of monolayer adherence was measured by staining with crystal violet and CyQUANT. The most sensitive test in each case was the assay related to the site of the direct damage, and measurement of the loss of monolayer adherence proved to be as sensitive assay as the damage-specific one. All the assays applied, exc...Continue Reading

References

May 15, 1989·Analytical Biochemistry·J A Cook, J B Mitchell
Oct 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A W KrauseW W Webb
Jan 1, 1984·Journal of Interferon Research·M Ito
May 23, 1998·Annual Review of Pharmacology and Toxicology·J C DavilaD Acosta
May 30, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R J Gonzalez, J B Tarloff
May 23, 2002·Photochemistry and Photobiology·Jean-Roch MeunierLaurent Marrot
Apr 1, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·M SchafferE Dühmke
Apr 8, 2003·Current Medicinal Chemistry. Anti-cancer Agents·M BinaschiF Animati
May 2, 2003·Nature Reviews. Cancer·Dennis E J G J DolmansRakesh K Jain
Dec 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M J GarleJ R Fry
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J R FryA Hatfield

❮ Previous
Next ❯

Citations

Dec 22, 2007·Nanomedicine·Jennifer B HallScott E McNeil
Jul 4, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Alexandra Vranyac-TramoundanasIvan A Sammut
Nov 22, 2011·Expert Opinion on Drug Metabolism & Toxicology·Arati SharmaGavin P Robertson
Dec 22, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Assunta De NisiMarco Bandini
Aug 19, 2011·Photochemistry and Photobiology·Mathias O Senge, Johan C Brandt
Jan 25, 2012·Basic & Clinical Pharmacology & Toxicology·Ian C WinburnJoanne C Harrison
Sep 17, 2013·Molecular and Cellular Endocrinology·Jessica F BriffaDeanne H Hryciw
Jan 18, 2007·Biochemical and Biophysical Research Communications·N CalonghiL Masotti
Sep 22, 2007·Journal of Toxicology and Environmental Health. Part a·Mustafa Al-KhalidiMary G Murphy
Apr 11, 2018·Journal of Cellular Biochemistry·Egle KukcinaviciuteArvydas Laurinavicius
Dec 31, 2005·Physical Review Letters·Adrian NeaguGabor Forgacs
Aug 10, 2017·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jian ZhangWeijian Guo
Dec 8, 2020·Frontiers in Immunology·Diego Pérez-StuardoSebastián Reyes-Cerpa
Aug 4, 2006·Cancer Research·Izabela Młynarczuk-BiałyUlrike Seifert
Apr 23, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wei HaYan-Ping Shi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.