Experimental transmission of US scrapie agent by nasal, peritoneal, and conjunctival routes to genetically susceptible sheep

Veterinary Pathology
Amir N HamirJ J Greenlee

Abstract

Scrapie is a naturally occurring fatal neurodegenerative disease of sheep and goats. This study documents incubation periods, pathologic findings, and distribution of abnormal prion proteins (PrP(Sc)) by immunohistochemistry in tissues of genetically susceptible sheep inoculated with US sheep scrapie agent. Four-month-old Suffolk lambs (QQ at codon 171) were inoculated by 1 of 3 different routes (nasal, peritoneal, and conjunctival) with an inoculum (No. 13-7) consisting of a pool of scrapie-affected sheep brains. Except for 3 sheep, all inoculated animals were euthanized when advanced clinical signs of scrapie were observed between 19 and 46 months postinoculation (MPI). Spongiform lesions in the brains and labeling of PrP(Sc) in central nervous system and lymphoid tissues were present in these sheep. One intranasally inoculated sheep euthanized at 12 MPI had presence of PrP(Sc) that was confined to the pharyngeal tonsil. These results indicate that the upper respiratory tract, specifically the pharyngeal tonsil, may serve as a portal of entry for prion protein in scrapie-infected environments.

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Citations

Jan 23, 2009·Journal of Virology·Cristiano CoronaCristina Casalone
Apr 17, 2009·Journal of Virology·Richard A BessenDaniel Gonzalez
Aug 28, 2009·Environmental Science & Technology·Samuel E SaundersShannon L Bartelt-Hunt
Mar 20, 2012·Cell and Tissue Research·Vinciane ToppetsNadine Antoine
Jan 24, 2012·Chemosphere·Samuel E SaundersShannon L Bartelt-Hunt
Feb 19, 2010·The Journal of General Virology·Nathaniel D DenkersEdward A Hoover
Apr 19, 2015·Veterinary Research·Kevin C GoughBen C Maddison
Aug 13, 2013·Journal of Neuroimmunology·Vinciane ToppetsNadine Antoine
Jan 21, 2011·PLoS Pathogens·Johannes HaybaeckAdriano Aguzzi
Apr 27, 2010·PLoS Pathogens·Richard A BessenJames A Wiley
Dec 30, 2008·Veterinary Pathology·A N HamirJ M Miller
Jul 20, 2018·Journal of Virology·Alison MarshallNeil A Mabbott
Sep 14, 2012·Journal of Virology·Anthony E KincaidJason C Bartz
Nov 24, 2012·Journal of Virology·Nathaniel D DenkersEdward A Hoover
Mar 19, 2009·PloS One·Nicholas J HaleyEdward A Hoover
Mar 25, 2011·Journal of Virology·Samuel E SaundersJason C Bartz
Aug 18, 2020·Emerging Topics in Life Sciences·Jacob I AyersStanley B Prusiner
Nov 20, 2009·Environmental Science & Technology·Samuel E SaundersShannon L Bartelt-Hunt
Aug 8, 2021·International Journal of Molecular Sciences·Sarah Vascellari, Aldo Manzin

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Methods Mentioned

BETA
light microscopy

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