Experimental verification of a predicted intronic microRNA in human NGFR gene with a potential pro-apoptotic function.

PloS One
Sepideh ParsiSeyed J Mowla

Abstract

Neurotrophins (NTs) are a family of secreted growth factor proteins primarily involved in the regulation of survival and appropriate development of neural cells, functioning by binding to their specific (TrkA, TtkB, and TrkC) and/or common NGFR receptor. NGFR is the common receptor of NTs, binding with low-affinity to all members of the family. Among different functions assigned to NGFR, it is also involved in apoptosis induction and tumorigenesis processes. Interestingly, some of the functions of NGFR appear to be ligand-independent, suggesting a probable involvement of non-coding RNA residing within the sequence of the gene. Here, we are reporting the existence of a conserved putative microRNA, named Hsa-mir-6165 [EBI accession#: FR873488]. Transfection of a DNA segment corresponding to the pre-mir-6165 sequence in Hela cell line caused the generation of mature exogenous mir-6165 (a ∼200,000 fold overexpression). Furthermore, using specific primers, we succeeded to detect the endogenous expression of mir-6165 in several glioma cell lines and glioma primary tumors known to express NGFR. Similar to the pro-apoptotic role of NGFR in some cell types, overexpression of pre-mir-6165 in U87 cell line resulted in an elevated rate of ...Continue Reading

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Citations

Nov 8, 2013·Functional & Integrative Genomics·Sadegh Azimzadeh JamalkandiAli Masoudi-Nejad
Mar 17, 2015·Cellular and Molecular Life Sciences : CMLS·Sadat DokanehiifardSeyed Javad Mowla
Apr 22, 2016·Toxicologic Pathology·June K DunnickKeith R Shockley
Aug 12, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ali Jason SalehSeyed Javad Mowla
Oct 6, 2018·Journal of Translational Medicine·Hargobinder KaurAli A Sultan
Oct 12, 2018·Journal of Cellular Biochemistry·Sadat Dokanehiifard, Bahram M Soltani
Sep 29, 2017·Journal of Molecular Neuroscience : MN·Maryam HassanlouSeyed Javad Mowla
Sep 28, 2018·Plant Methods·Umidjon ShapulatovAlexander R van der Krol
Jan 16, 2021·International Journal of Molecular Sciences·Margarita A SazonovaIgor A Sobenin
Mar 7, 2021·Journal of Cellular and Molecular Medicine·Sadat Dokaneheifard, Bahram M Soltani

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Datasets Mentioned

BETA
FR873489

Methods Mentioned

BETA
PCR
transfection
biopsies
flow cytometry
profiler

Software Mentioned

GENEMANIA
Pmirp
GraphPad Prism
miR
blast search
RNAHybride
DIANA
Mireval
MatureBayes
SCC profiler

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