Experimentally Induced Bladder Permeability Evokes Bladder Afferent Hypersensitivity in the Absence of Inflammation

Frontiers in Neuroscience
Luke GrundyStuart M Brierley

Abstract

Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic urological condition characterised by urinary urgency, frequency and pelvic pain, that significantly impacts the quality of life for ∼5% of women. Bladder sensation is coordinated by primary afferent sensory neurons that innervate the bladder wall, translating bladder stretch into signals that travel to the brain via the spinal cord. Whilst the pathophysiology of IC/BPS remains unknown, an increase in the permeability of the bladder urothelium has been proposed as an initiating cause. Here we experimentally increased bladder permeability and tracked bladder afferent sensitivity for up to 28 days. We found that one day after increasing bladder epithelial permeability with in vivo bladder infusion of protamine sulfate, mechanosensitive bladder afferents exhibited significant hypersensitivity to bladder filling. This mechanical hypersensitivity was characterised by significantly increased peak afferent firing rates and a decrease in the activation threshold of individual afferents. Bladder afferent hypersensitivity occurred in the absence of inflammation and changes in bladder muscle compliance, indicating a direct sensitisation of peripheral afferent endings. Bladd...Continue Reading

References

Feb 1, 1996·Clinical and Experimental Pharmacology & Physiology·M MichaelisW Jäenig
Jul 1, 1996·Pain·Natalia Dmitrieva, Stephen B McMahon
Jul 12, 2002·American Journal of Physiology. Renal Physiology·John LavelleMark L Zeidel
Jan 11, 2007·Nature Clinical Practice. Urology·Lori A Birder, William C de Groat
May 21, 2008·Nature Reviews. Neuroscience·Clare J FowlerWilliam C de Groat
Aug 6, 2009·Handbook of Experimental Pharmacology·William C de Groat, Naoki Yoshimura
Nov 24, 2012·The Journal of Urology·Kristina M StemlerIndira U Mysorekar
Apr 17, 2013·Physiological Reviews·Lori Birder, Karl-Erik Andersson
Apr 17, 2014·BioMed Research International·Eric J GonzalezMargaret A Vizzard
May 9, 2014·International Journal of Urology : Official Journal of the Japanese Urological Association·Naoki YoshimuraTeruyuki Ogawa
Jun 6, 2014·BioMed Research International·Susan K KeayToby C Chai
Jul 9, 2014·Nature Reviews. Gastroenterology & Hepatology·Stuart M Brierley, David R Linden
Mar 18, 2015·The Journal of Urology·Beverley Greenwood-Van MeerveldRobert Hurst
Apr 9, 2015·Reviews of Physiology, Biochemistry and Pharmacology·Eva LasičMateja Erdani Kreft
Jan 22, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Caihong WangIndira U Mysorekar
Jun 1, 2017·ENeuro·Nicolas MontalbettiMarcelo D Carattino
Nov 28, 2017·The Journal of Comparative Neurology·Nick J SpencerVladimir P Zagorodnyuk
Dec 16, 2017·Cell Reports·Vincenzo PratoStefan G Lechner
Jan 18, 2018·Archives of Biochemistry and Biophysics·Yasuaki Aratani
Feb 27, 2018·The International Journal of Biochemistry & Cell Biology·Mahsa SadeghiStuart M Brierley
May 23, 2018·The Journal of Pharmacology and Experimental Therapeutics·Ehsan N MohammadiBeverley Greenwood-Van Meerveld
Jun 14, 2018·American Journal of Physiology. Renal Physiology·Luke GrundyDavid Grundy
Nov 1, 2018·Annual Review of Physiology·Luke GrundyStuart M Brierley
Jan 9, 2019·Frontiers in Neuroscience·Luke GrundyStuart M Brierley
Sep 15, 2019·The Journal of Physiology·N KonthapakdeeD M Daly
Dec 4, 2019·American Journal of Physiology. Renal Physiology·Luke GrundyStuart M Brierley
Apr 7, 2020·Experimental Neurology·Wenbin GuoJonathan M Beckel

❮ Previous
Next ❯

Methods Mentioned

BETA
chip
dissection
Assay

Software Mentioned

GraphPad Prism
Spike

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.