Experimentally-induced maternal hypothyroidism alters enzyme activities and the sensorimotor cortex of the offspring rats

Molecular and Cellular Endocrinology
Juliana Tonietto DominguesAriane Zamoner

Abstract

In this study, we used an experimental model of congenital hypothyroidism to show that deficient thyroid hormones (TH) disrupt different neurochemical, morphological and functional aspects in the cerebral cortex of 15-day-old offspring. Our results showing decreased glutamine synthetase (GS) activity and Ca2+ overload in the cerebral cortex of hypothyroid pups suggest misregulated glutamate metabolism associated with developmentally induced TH deficiency. The 14C-MeAIB accumulation indicates upregulated System A activity and glutamine uptake by neurons. Energy metabolism in hypothyroid cortical slices was preserved, as demonstrated by unaltered glucose metabolism. We also found upregulated acetylcholinesterase activity, depleting acetylcholine from the synaptic cleft, pointing to disrupted cholinergic system. Increased reactive oxygen species (ROS) generation, lipid peroxidation, glutathione (GSH) depletion, which were associated with glutathione peroxidase, superoxide dismutase and gamma-glutamyltransferase downregulation suggest redox imbalance. Disrupted astrocyte cytoskeleton was evidenced by downregulated and hyperphosphorylated glial fibrillary acidic protein (GFAP). Morphological and structural characterization of the se...Continue Reading

References

Jan 1, 1977·Diabetologia·H J Gundersen, R Osterby
Jan 1, 1985·Methods in Enzymology·I Carlberg, B Mannervik
Jan 1, 1984·Methods in Enzymology·L Flohé, W A Günzler
Jan 1, 1984·Methods in Enzymology·H Aebi
Jan 1, 1984·Methods in Enzymology·R P Bird, H H Draper
Feb 1, 1995·Protein Engineering·A C WallaceJ M Thornton
Aug 1, 1997·Endocrinology·H L SchwartzJ H Oppenheimer
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·A Guadaño-FerrazJ Bernal
Oct 13, 1998·The Journal of Clinical Endocrinology and Metabolism·A CassioP Pirazzoli
Jan 27, 1999·Thyroid : Official Journal of the American Thyroid Association·M Polak
Feb 22, 2000·The Journal of the American Board of Family Practice·T J Caffrey
Jan 6, 2001·Frontiers in Neuroendocrinology·G W Anderson
Jul 31, 2001·Biochimica Et Biophysica Acta·K Das, G B Chainy
Oct 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·A G TrentinV Moura Neto
Apr 9, 2002·Journal of Endocrinological Investigation·J Bernal
Dec 18, 2002·Annals of the New York Academy of Sciences·Dale A Dickinson, Henry Jay Forman
Jan 15, 2003·The Journal of Endocrinology·C MenendezF F Casanueva
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Michael J BerridgeH Llewelyn Roderick
Dec 4, 2003·Thyroid : Official Journal of the American Thyroid Association·Juan BernalBeatriz Morte
Apr 30, 2004·Journal of Computational Chemistry·Junmei WangDavid A Case
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Sep 30, 2004·Neurochemical Research·Kajari Das, G B N Chainy
Jan 26, 2005·Journal of Chemical Information and Modeling·John J Irwin, Brian K Shoichet
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Nov 23, 2005·Journal of Neuroscience Research·Asmita DasguptaPranab Kumar Sarkar
Jan 10, 2006·Methods in Enzymology·Hongqiao ZhangJinah Choi
May 2, 2006·The Journal of Endocrinology·Andréa Gonçalves Trentin
Feb 20, 2007·Molecular and Cellular Endocrinology·Ariane ZamonerRegina Pessoa-Pureur
Mar 6, 2007·Cellular and Molecular Neurobiology·Ariane ZamonerRegina Pessoa-Pureur
Nov 23, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Osama M AhmedR G Ahmed
Mar 5, 2008·Journal of Molecular Endocrinology·Ariane ZamonerFátima Regina Mena Barreto Silva

❮ Previous
Next ❯

Citations

Feb 24, 2019·International Journal of Molecular Sciences·Mitsuhiro MoritaMami Noda
Jan 10, 2021·Molecular and Cellular Endocrinology·Juliane de Souza CardosoRejane Giacomelli Tavares

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Related Papers

Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]
Omeed AhadiatAshley Wysong
Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology
Srikanta Jena
© 2022 Meta ULC. All rights reserved