Experimentally induced metamorphosis in highly regenerative axolotl (ambystoma mexicanum) under constant diet restructures microbiota

Scientific Reports
Turan DemircanSüleyman Yıldırım

Abstract

Axolotl (Ambystoma mexicanum) is a critically endangered salamander species and a model organism for regenerative and developmental biology. Despite life-long neoteny in nature and in captive-bred colonies, metamorphosis of these animals can be experimentally induced by administering Thyroid hormones (THs). However, microbiological consequences of this experimental procedure, such as host microbiota response, remain largely unknown. Here, we systematically compared host bacterial microbiota associated with skin, stomach, gut tissues and fecal samples, between neotenic and metamorphic axolotls based on 16S rRNA gene sequences. Our results show that distinct bacterial communities inhabit individual organs of axolotl and undergo substantial restructuring through metamorphosis. Skin microbiota among others, shifted sharply, as highlighted by a major transition from Firmicutes-enriched to Proteobacteria-enriched relative abundance and precipitously decreased diversity. Fecal microbiota of neotenic and metamorphic axolotl shared relatively higher similarity, suggesting that diet continues to shape microbiota despite fundamental transformations in the host digestive organs. We also reproduced the previous finding on reduction in regen...Continue Reading

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Citations

Oct 9, 2019·Endocrinology·Marietta R EasterlingErica J Crespi
Jan 29, 2020·Molecular Ecology Resources·Jia ZhouLee Ann Rollins
Aug 24, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Veronika OlejnickovaMartina Gregorovicova
Nov 30, 2019·Gerontology·Warren A VieiraCatherine D McCusker

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Datasets Mentioned

BETA
MG518658
MG519278

Methods Mentioned

BETA
amputation
biopsy
dissection
electrophoresis
PCR

Software Mentioned

PRIMER6
jvenn
SortMeRNA
BLAST
MicrobiomeAnalyst
R
Canonical Analysis of Principal coordinates ( CAP
Mothur
Phylogenetic Investigations of Communities by Reconstruction (...
PERMDISP

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