Explaining combinatorial effects of mycotoxins Deoxynivalenol and Zearalenone in mice with urinary metabolomic profiling

Scientific Reports
Jian JiXiulan Sun

Abstract

Urine metabolic profiling of mice was conducted utilizing gas chromatography-mass spectrometry (GC-MS) to investigate the combinatory effect of mycotoxins deoxynivalenol (DON) and zearalenone (ZEN) on the metabolism of the mice. Experiments were conducted by means of five-week-old mice which were individually exposed to 2 mg/kg DON, 20 mg/kg ZEN and the mixture of DON and ZEN (2 mg/kg and 20 mg/kg, respectively). The intragastric administration was applied for three weeks and urine samples were collected for metabolic analysis. Univariate and multivariate analysis were applied to data matrix processing along with respective pathway analysis by MetaMapp and CytoScape. The results showed that the combined DON and ZEN administration resulted in lower significant changes, compared to the individual mycotoxin treated groups verified by heatmap. Metabolic pathways network mapping indicated that the combined mycotoxins treated groups showed a little effect on the metabolites in most pathways, especially in glucose metabolism and its downstream amino acid metabolism. In glucose metabolism, the content of galactose, mannitol, galactonic acid, myo-inositol, tagatose was drastically down-regulated. Furthermore, the organic acids, pyruvate...Continue Reading

References

Nov 1, 1977·Applied and Environmental Microbiology·D M ForsythJ Tuite
Jan 1, 1990·Journal of Inherited Metabolic Disease·S Segal, S Rogers
Mar 1, 1986·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J H ForsellJ J Pestka
Apr 1, 1987·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J J PestkaJ H Forsell
Feb 1, 1987·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J H ForsellJ J Pestka
Jun 1, 1969·Journal of Bacteriology·L N Ornston, M K Ornston
May 1, 1996·Journal of Toxicology and Environmental Health·B A RotterJ J Pestka
Mar 15, 2000·Biological Psychiatry·M E WinsbergT A Ketter
Sep 25, 2003·Annual Review of Plant Biology·Wolfram Weckwerth
Sep 3, 2004·Toxicology Letters·D Parent-Massin
Oct 8, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M E GouzeP Galtier
Oct 20, 2005·Journal of Bioscience and Bioengineering·M J TaherzadehG Lidén
Oct 13, 2006·Methods in Molecular Biology·Oliver Fiehn, Tobias Kind
Oct 19, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Abdellah ZinedineJordi Mañes
Feb 24, 2007·Analytical Biochemistry·Tobias KindRobert H Weiss
Aug 28, 2007·International Journal of Food Microbiology·John L Richard
Nov 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ralph J DeBerardinisCraig B Thompson
Feb 3, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Bethanne M WarrackDieter M Drexler
May 12, 2009·Nucleic Acids Research·Jianguo XiaDavid S Wishart
Jul 20, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ji-Yeon YuJeong-Chae Lee
Sep 14, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Aihua ZhangXijun Wang
Apr 8, 2015·Bioinformatics·Dmitry GrapovOliver Fiehn
Apr 17, 2015·Mass Spectrometry Reviews·Mona M KhamisAnas El-Aneed
May 6, 2015·Nature Methods·Hiroshi TsugawaMasanori Arita
Aug 18, 2016·Toxicon : Official Journal of the International Society on Toxinology·Jian JiXiulan Sun
Sep 8, 2016·Current Protocols in Bioinformatics·Jianguo Xia, David S Wishart

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Citations

Mar 8, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Imourana Alassane-KpembiIsabelle P Oswald
Mar 3, 2021·Food Research International·Ajibola Bamikole OyedejiOluwafemi Ayodeji Adebo
Mar 20, 2021·Analytica Chimica Acta·M Hernández-MesaG Dervilly

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Methods Mentioned

BETA
PCA

Software Mentioned

R
RandomForest
MS DIAL
Shimadzu GCMS PostRun
OPLS
CytoScape
MetaboAnalyst
DA
R i386
MetaMapp

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