Explicit targeting of transformed cells by VSV in ovarian epithelial tumor-bearing Wv mouse models.

Gynecologic Oncology
Callinice D Capo-chichiXiang-Xi Xu

Abstract

Current treatment options for epithelial ovarian cancer are limited and therapeutic development for recurrent and drug-resistant ovarian cancer is an urgent agenda. We investigated the potential use of genetically engineered Vesicular Stomatitis Virus (VSV) to treat ovarian cancer patients who fail to respond to available therapies. Specifically, we examined the toxicity to hosts and specificity of targeting ovarian tumors using a Wv ovarian tumor model. We first tested recombinant VSV for oncolytic activity in a panel of human ovarian epithelial cancer, immortalized, and primary ovarian surface epithelial cells in culture. Then, we tested VSV oncolytic therapy using the immune competent Wv mice that develop tubular adenomas, benign tumor lesions derived from ovarian surface epithelial cells. The expression of GFP encoded by the recombinant VSV genome was detected in about 5% of primary ovarian surface epithelial cells (3 lines) up to 30 days without significantly altering the growth pattern of the cells, suggesting the lack of toxicity to the normal ovarian surface epithelial cells. However, VSV-GFP was detected in the majority (around 90%) of cells that are either "immortalized" by SV40 antigen expression or cancer lines. Som...Continue Reading

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Jul 8, 2009·Molecular and Cellular Biology·Callinice D Capo-chichiXiang-Xi Xu

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Citations

Nov 20, 2013·Gynecologic Oncology·Yu-Ping LiuJamie N Bakkum-Gamez
Jan 31, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Alexander MuikDorothee von Laer
Apr 2, 2016·ILAR Journal·Theresa FallsMarie-Claude Bourgeois-Daigneault

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