Abstract
Current treatment options for epithelial ovarian cancer are limited and therapeutic development for recurrent and drug-resistant ovarian cancer is an urgent agenda. We investigated the potential use of genetically engineered Vesicular Stomatitis Virus (VSV) to treat ovarian cancer patients who fail to respond to available therapies. Specifically, we examined the toxicity to hosts and specificity of targeting ovarian tumors using a Wv ovarian tumor model. We first tested recombinant VSV for oncolytic activity in a panel of human ovarian epithelial cancer, immortalized, and primary ovarian surface epithelial cells in culture. Then, we tested VSV oncolytic therapy using the immune competent Wv mice that develop tubular adenomas, benign tumor lesions derived from ovarian surface epithelial cells. The expression of GFP encoded by the recombinant VSV genome was detected in about 5% of primary ovarian surface epithelial cells (3 lines) up to 30 days without significantly altering the growth pattern of the cells, suggesting the lack of toxicity to the normal ovarian surface epithelial cells. However, VSV-GFP was detected in the majority (around 90%) of cells that are either "immortalized" by SV40 antigen expression or cancer lines. Som...Continue Reading
References
Mar 1, 1990·Genes & Development·A D ReithA Bernstein
Mar 1, 1988·Journal of Cellular Physiology·C H Siemens, N Auersperg
Sep 23, 2000·Journal of Virology·D F StojdlJ C Bell
Mar 10, 2001·Journal of Virology·S BalachandranG N Barber
Dec 26, 2001·Journal of Virology·Marilyn FernandezGlen N Barber
Jul 30, 2003·Journal of Virology·Masatsugu ObuchiGlen N Barber
Jan 30, 2004·Cancer Cell·Siddharth Balachandran, Glen N Barber
May 4, 2004·Trends in Molecular Medicine·Brian D LichtyJohn C Bell
Nov 27, 2004·Cancer Gene Therapy·Oliver EbertSavio L C Woo
Dec 25, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Katsunori ShinozakiSavio L C Woo
Jan 27, 2005·Viral Immunology·Glen N Barber
Nov 19, 2005·Nature Reviews. Cancer·Kelley A ParatoJohn C Bell
Nov 22, 2005·Oncogene·Glen N Barber
Feb 15, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Elizabeth Kelly, Stephen J Russell
Mar 17, 2007·Cancer Research·Rosa Maria DiazRichard G Vile
Jan 25, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Dirk M Nettelbeck
Mar 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lainie P MartinRussell J Schilder
Jun 19, 2008·Cancer Research·Boris A HadaschikPaul S Rennie
Apr 1, 2009·Seminars in Oncology·Lainie P Martin, Russell J Schilder
May 23, 2009·Nature Reviews. Cancer·Robert C BastGordon B Mills
Jul 8, 2009·Molecular and Cellular Biology·Callinice D Capo-chichiXiang-Xi Xu
Citations
Nov 20, 2013·Gynecologic Oncology·Yu-Ping LiuJamie N Bakkum-Gamez
Jan 31, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Alexander MuikDorothee von Laer
Aug 5, 2011·Journal of Virology·Joshua F Heiber, Glen N Barber
Nov 9, 2011·Chinese Journal of Cancer·Joshua F HeiberGlen N Barber
Apr 2, 2016·ILAR Journal·Theresa FallsMarie-Claude Bourgeois-Daigneault
Oct 16, 2016·Molecular Therapy Oncolytics·Catherine DoldDorothee von Laer
May 9, 2014·Cancer Research·Alexander MuikDorothee von Laer