Exploitation of KESTREL to identify NDRG family members as physiological substrates for SGK1 and GSK3

The Biochemical Journal
James T MurrayPhilip Cohen


We detected a protein in rabbit skeletal muscle extracts that was phosphorylated rapidly by SGK1 (serum- and glucocorticoid-induced kinase 1), but not by protein kinase Ba, and identified it as NDRG2 (N-myc downstream-regulated gene 2). SGK1 phosphorylated NDRG2 at Thr330, Ser332 and Thr348 in vitro. All three residues were phosphorylated in skeletal muscle from wild-type mice, but not from mice that do not express SGK1. SGK1 also phosphorylated the related NDRG1 isoform at Thr328, Ser330 and Thr346 (equivalent to Thr330, Ser332 and Thr348 of NDRG2), as well as Thr356 and Thr366. Residues Thr346, Thr356 and Thr366 are located within identical decapeptide sequences GTRSRSHTSE, repeated three times in NDRG1. These threonines were phosphorylated in NDRG1 in the liver, lung, spleen and skeletal muscle of wild-type mice, but not in SGK1-/- mice. Knock-down of SGK1 in HeLa cells using small interfering RNA also suppressed phosphorylation of the threonine residues in the repeat region of NDRG1. The phosphorylation of NDRG1 by SGK1 transformed it into an excellent substrate for GSK3 (glycogen synthase kinase 3), which could then phosphorylate Ser342, Ser352 and Ser362 in the repeat region. Incubation of HeLa cells with the specific GSK...Continue Reading


Jul 8, 1999·Nature Medicine·J S Sebolt-LeopoldA R Saltiel
Jun 1, 2000·American Journal of Human Genetics·L KalaydjievaP K Thomas
May 2, 2001·Journal of Inorganic Biochemistry·M A ZorodduMax Costa
Nov 7, 2001·The Journal of Biological Chemistry·Peter M SnyderBrittany C Thomas
May 28, 2002·Neurogenetics·Philipp BergerUeli Suter
Jun 20, 2002·The Journal of Biological Chemistry·Sheerazed BoulkrounNathalie Courtois-Coutry
Nov 15, 2002·Histochemistry and Cell Biology·Pascale LachatFred T Bosman
Jan 22, 2003·The Biochemical Journal·Jenny BainPhilip Cohen
Mar 22, 2003·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·G L FirestoneBridget A O'Keeffe
Mar 22, 2003·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Florian LangVolker Vallon
Jul 26, 2003·The EMBO Journal·Simon MortonPhilip Cohen
Aug 13, 2003·The EMBO Journal·Barry J CollinsDario R Alessi
Aug 21, 2003·The Journal of Biological Chemistry·Ratan BhatJesús Avila
Oct 3, 2003·The Journal of Biological Chemistry·Sun Sik BaeMorris J Birnbaum
Feb 27, 2004·The Journal of Biological Chemistry·James G BurchfieldCarsten Schmitz-Peiffer
Apr 15, 2004·Molecular and Cellular Biology·Tomohiko OkudaToshiyuki Miyata
Jun 3, 2004·Nature Reviews. Drug Discovery·Philip Cohen, Michel Goedert
Jul 13, 2004·The Journal of Biological Chemistry·Kyung-Tae KimSam W Lee
Oct 6, 2004·The Biochemical Journal·James T MurrayPhilip Cohen


Mar 8, 2006·Cell and Tissue Research·Xiao-Lan HuLibo Yao
Dec 16, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Mei-Sze ChuaSamuel K So
Feb 27, 2014·Nature Communications·Shingo NakahataKazuhiro Morishita
Dec 23, 2009·Nature Reviews. Molecular Cell Biology·Laura R PearceDario R Alessi
Feb 12, 2009·The Journal of Biological Chemistry·Ke NingMichael L J Ashford
Jan 19, 2007·Molecular Biology of the Cell·Mónica AlvarezSusana de la Luna
Mar 23, 2007·Molecular Biology of the Cell·Maria Francisca ArteagaCecilia M Canessa
Mar 19, 2008·Molecular and Cellular Biology·Jose R BayascasDario R Alessi
Feb 8, 2006·Annual Review of Physiology·Johannes LoffingOlivier Staub
Jan 12, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Alexei DiakovChristoph Korbmacher
Sep 22, 2012·Journal of Translational Medicine·Xinrong PengMengchao Wu
Apr 7, 2011·Experimental & Molecular Medicine·Chang Hoon Cho
Apr 2, 2014·The Journal of Cell Biology·Bradley A HellerJames L Salzer
Apr 30, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·I M AparicioP Lonergan
Aug 7, 2013·Pharmacology & Therapeutics·Margaret K KingEléonore Beurel
Jun 2, 2011·International Journal of Alzheimer's Disease·Calum Sutherland
Oct 3, 2014·Molecular Biology of the Cell·Min ZengJie Chen
May 24, 2011·Leukemia & Lymphoma·Joseph Gera, Alan Lichtenstein
Feb 18, 2016·Journal of the American Society of Nephrology : JASN·Jinlong LuoJizhong Cheng
Oct 6, 2010·Growth Factors·Maressa A BruhnKaren E Sheppard
Jan 17, 2013·Biochemical and Biophysical Research Communications·Xinrong PengQijun Qian
Feb 11, 2009·The Journal of Physiology·Victoria C FolettaKen R Walder
Jun 12, 2012·Cell Calcium·Florian LangEkaterina Shumilina
Oct 27, 2015·Frontiers in Cellular Neuroscience·Veronika MatschkeNathalie Strutz-Seebohm
Oct 11, 2008·International Journal of Cancer. Journal International Du Cancer·Young Jun KimJae Wha Kim
Feb 1, 2013·Neuropathology and Applied Neurobiology·P SahinAtticus H Hainsworth
Sep 5, 2015·Experimental Physiology·Thorbjørn H ChristensenRobert W Hunter
Oct 31, 2006·Biochimica Et Biophysica Acta·Corinne M SpickettWalter Kolch
Dec 3, 2014·Molecular Cell·Jessica A GasserAlex Toker
Sep 6, 2015·Acta Biochimica Et Biophysica Sinica·Kaifeng LinYan Li
Sep 18, 2015·Cell Death & Disease·D PastoreD Lauro
Aug 8, 2014·Journal of Neurochemistry·Elizabeth A HellerMichelle S Mazei-Robison
May 1, 2009·The Biochemical Journal·Juan M García-MartínezDario R Alessi
Mar 19, 2011·The Biochemical Journal·Laura R PearceDario R Alessi
Oct 27, 2012·Journal of Proteomics·James D R KnightAnne-Claude Gingras
Nov 23, 2010·The Biochemical Journal·Ayaz NajafovDario R Alessi
Apr 16, 2013·The Biochemical Journal·Eeva M SommerDario R Alessi
May 19, 2006·Expert Opinion on Therapeutic Targets·Sheelagh Frame, Daniella Zheleva
Jun 26, 2015·Scientific Data·Munazah AndrabiNick R Love
Mar 25, 2014·European Journal of Pharmacology·Noor A S IsmailStuart M Wilson
Jun 29, 2011·Biochemical and Biophysical Research Communications·Catherine McCaigJames T Murray
May 5, 2010·Biochemical and Biophysical Research Communications·Seung-Chul ChoiJae Wha Kim
Aug 6, 2010·Blood·Bao HoangAlan Lichtenstein
Dec 5, 2014·Pflügers Archiv : European journal of physiology·Katrina J BingerMarkus Kleinewietfeld
Aug 16, 2016·International Journal of Molecular Sciences·Yiyun LouFan Jin
Feb 16, 2005·FEBS Letters·James T MurrayPhilip Cohen
Apr 6, 2013·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Yurong TaoLibo Yao
Sep 26, 2013·Molecular & Cellular Proteomics : MCP·Dietmar KültzRomina Sacchi
Apr 8, 2014·Nature Immunology·Emily B HeikampJonathan D Powell
Aug 26, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Teresa F AckermannFlorian Lang
Jan 10, 2018·Journal of Neurochemistry·Thuong Manh LeOsamu Hori
Nov 5, 2014·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yoshitaka Taketomi
Feb 17, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Elisenda Alsina-SanchísFrancesc Viñals
Apr 19, 2006·Journal of Cellular Biochemistry·Maude Tessier, James R Woodgett
May 24, 2008·Nature·Qi-Long YingAustin Smith
Aug 24, 2005·The Journal of Biological Chemistry·Maria Emanuela CuomoSibylle Mittnacht
Jan 24, 2007·The Journal of Biological Chemistry·Rainer MussmannMatthias Austen
Jul 27, 2007·The Journal of Biological Chemistry·Mark O WielpützRobert Rauh
May 19, 2019·Journal of Experimental & Clinical Cancer Research : CR·Silvia MatteoniMarco G Paggi
Jul 18, 2019·American Journal of Physiology. Renal Physiology·Venkatesh DeshpandeMark A Knepper
Jul 18, 2019·American Journal of Physiology. Cell Physiology·Marni D Boppart, Ziad S Mahmassani
Mar 7, 2014·American Journal of Physiology. Cell Physiology·Davis BradfordTrairak Pisitkun
Oct 6, 2004·The Biochemical Journal·James T MurrayPhilip Cohen
Feb 19, 2020·Expert Opinion on Therapeutic Targets·Inés MaestroAna Martinez
May 26, 2017·Oncotarget·Shuye TianChristopher G Proud
Jul 30, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Veerle MelotteManon van Engeland
Dec 21, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Catherine McCaigAtticus H Hainsworth
Sep 10, 2020·Scientific Reports·Marie A DoyleMichelle S Mazei-Robison
Jul 1, 2015·Oncotarget·Goldie Yuan Lam LuiDes R Richardson
Feb 28, 2017·Biomarkers in Medicine·Andrea Nicolini
Mar 25, 2017·Scientific Reports·Vassilios J BezzeridesAnthony Rosenzweig
Mar 19, 2020·Stem Cell Reviews and Reports·Guofang ChenXiaoping Wan
Nov 30, 2005·The Journal of Biological Chemistry·Baosheng ChenYoel Sadovsky
Apr 21, 2007·The Biochemical Journal·Helene MaccarioNick R Leslie
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoshitaka TaketomiIchiro Kudo
Oct 25, 2017·Cancers·Aikaterini Emmanouilidi, Marco Falasca
Jun 15, 2018·Breast Cancer Research : BCR·Christopher J SevinskyDouglas S Conklin
May 10, 2020·Nature Communications·Ling RaoLydia W T Cheung
Jul 31, 2020·Therapeutic Advances in Medical Oncology·Ruizhe ZhuTaiping Zhang
Jul 8, 2008·Acta Biochimica Et Biophysica Sinica·Libo YaoXuewu Liu
Dec 13, 2005·The Biochemical Journal·Philip Cohen, Axel Knebel
Oct 12, 2018·Current Medicinal Chemistry·Mentor SopjaniMiribane Dërmaku-Sopjani
Dec 14, 2019·Cellular and Molecular Life Sciences : CMLS·Xin LiLize Xiong
Sep 24, 2005·The Biochemical Journal·Christopher LipinaCalum Sutherland
Sep 14, 2007·The Biochemical Journal·Jenny BainPhilip Cohen
Feb 9, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yuanzhong WangShiuan Chen
Sep 2, 2017·Pflügers Archiv : European journal of physiology·Morag K MansleyMarko Bertog
Aug 10, 2017·Cancer Research·Frank CichockiJeffrey S Miller
Sep 28, 2018·Molecular Cancer Research : MCR·Evan J GreenawaltChristine M Eischen
Mar 24, 2021·ACS Chemical Biology·Svenja WiechmannBernhard Kuster
May 24, 2007·ACS Chemical Biology·Lucy M ElphickDavid J Mann

Related Concepts

RPS6KA1 protein, human
SGK1 protein, rat
Bacteriological Cure
NDRG2 protein, human
S6K1, mouse
Antibody Specificity
Enzyme Inhibitors
Genes, Reiterated
HeLa Cells
Nucleolar Proteins

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.