Exploitation of syndecan-1 shedding by Pseudomonas aeruginosa enhances virulence

Nature
P W ParkM Bernfield

Abstract

Cell-surface heparan sulphate proteoglycans (HSPGs) are ubiquitous and abundant receptors/co-receptors of extracellular ligands, including many microbes. Their role in microbial infections is poorly defined, however, because no cell-surface HSPG has been clearly connected to the pathogenesis of a particular microbe. We have previously shown that Pseudomonas aeruginosa, through its virulence factor LasA, enhances the in vitro shedding of syndecan-1-the predominant cell-surface HSPG of epithelia. Here we show that shedding of syndecan-1 is also activated by P. aeruginosa in vivo, and that the resulting syndecan-1 ectodomains enhance bacterial virulence in newborn mice. Newborn mice deficient in syndecan-1 resist P. aeruginosa lung infection but become susceptible when given purified syndecan-1 ectodomains or heparin, but not when given ectodomain core protein, indicating that the ectodomain's heparan sulphate chains are the effectors. In wild-type newborn mice, inhibition of syndecan-1 shedding or inactivation of the shed ectodomain's heparan sulphate chains prevents lung infection. Our findings uncover a pathogenetic mechanism in which a host response to tissue injury-syndecan-1 shedding-is exploited to enhance microbial virulen...Continue Reading

References

Mar 1, 1992·The Journal of Cell Biology·M T ShiehP G Spear
Jun 1, 1992·The Journal of Cell Biology·S J PancakeS L Hoffman
Apr 1, 1995·Infection and Immunity·H TangA Prince
Jan 1, 1997·Infection and Immunity·K S Rostand, J D Esko
Sep 1, 1996·The Journal of Experimental Medicine·F D MenozziC Locht
Jun 6, 1997·The Journal of Biological Chemistry·S V SubramanianM Bernfield
Jun 13, 1998·The Journal of Biological Chemistry·V KainulainenM Bernfield
Aug 12, 1998·American Journal of Respiratory Cell and Molecular Biology·E C Crouch
Oct 8, 1998·American Journal of Respiratory Cell and Molecular Biology·A M LeVineT R Korfhagen
Feb 2, 1999·The Journal of Clinical Investigation·R BalsJ M Wilson
Jun 15, 1999·Pediatric Research·K M Huttner, C L Bevins
Feb 1, 2000·The Journal of Biological Chemistry·P W ParkM Bernfield
Jun 29, 2000·Annual Review of Biochemistry·M BernfieldM Zako
Jul 11, 2000·Nature Genetics·C M AlexanderM Bernfield
Aug 10, 2000·The Journal of Biological Chemistry·P W ParkM Bernfield

❮ Previous
Next ❯

Citations

Oct 9, 2002·Medicinal Research Reviews·Koichi Honke, Naoyuki Taniguchi
Dec 18, 2001·Medicinal Research Reviews·Jian Liu, Suzanne C Thorp
Oct 19, 2012·Applied Microbiology and Biotechnology·Piotr SzwedaSlawomir Milewski
Oct 22, 2011·Cancer Metastasis Reviews·Isabelle M BerquinYong Q Chen
Aug 9, 2003·Current Allergy and Asthma Reports·Richard L Gallo, Victor Nizet
Feb 2, 2010·Expert Reviews in Molecular Medicine·Allison H Bartlett, Pyong Woo Park
Apr 25, 2002·Molecular Microbiology·Franco D MenozziCamille Locht
Jul 17, 2009·The Journal of Biological Chemistry·Andrea BabelovaLiliana Schaefer
Dec 4, 2010·The Journal of Biological Chemistry·Atsuko HayashidaPyong Woo Park
Feb 3, 2012·The Journal of Biological Chemistry·Vishnu C RamaniRalph D Sanderson
May 24, 2008·The Journal of Biological Chemistry·Kazutaka HayashidaPyong Woo Park
Oct 30, 2008·The Journal of Biological Chemistry·Kazutaka HayashidaPyong Woo Park
Sep 6, 2002·Glycobiology·Janet M Trowbridge, Richard L Gallo
Mar 28, 2009·Critical Care Medicine·Jeffrey L VeesenmeyerJordi Rello
Aug 12, 2010·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Anthony J SmithAshley T Haase
Jun 22, 2011·Cold Spring Harbor Perspectives in Biology·Stephane SarrazinJeffrey D Esko
Jan 9, 2007·Respirology : Official Journal of the Asian Pacific Society of Respirology·Masaru HasegawaMasaharu Nishimura
Jun 28, 2006·Antimicrobial Agents and Chemotherapy·Joanne E ThwaiteTimothy P Atkins
Apr 5, 2002·Clinical Microbiology Reviews·Jeffrey B LyczakGerald B Pier
Dec 3, 2008·Infection and Immunity·Miriam J BaronLawrence C Madoff
Nov 22, 2005·Infection and Immunity·Allan HaynesKendra P Rumbaugh
Jul 10, 2013·Journal of Bacteriology·Alicia E Ballok, George A O'Toole
Jun 30, 2006·Annual Review of Cell and Developmental Biology·Hannes E Bülow, Oliver Hobert
Jan 24, 2007·Physiological Reviews·Kendra J GreenleeFarrah Kheradmand
Apr 19, 2003·American Journal of Respiratory and Critical Care Medicine·Stanley C H ChanMary S M Ip
Feb 8, 2005·American Journal of Respiratory and Critical Care Medicine·Ruxana T SadikotAlice S Prince
Oct 7, 2009·The Journal of Clinical Investigation·Kristin I StanfordJeffrey D Esko
Feb 9, 2006·BMC Microbiology·Taissia G PopovaSerguei G Popov
Aug 18, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Anna S Eriksson, Dorothe Spillmann
Mar 24, 2009·PloS One·Ligong Chen, Ralph D Sanderson
Jul 25, 2009·Cellular and Molecular Life Sciences : CMLS·Catherine Kirn-SafranDaniel D Carson
May 1, 2015·Antibiotics·Victor I Band, David S Weiss
Dec 16, 2006·The Journal of Eukaryotic Microbiology·Shao-Hung WangChao-Hung Lee
Jun 9, 2009·Journal of Medical Microbiology·Joanne E ThwaiteHelen S Atkins
Dec 24, 2014·Microbiology·Kari L AoyagiMark A Fisher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.