Exploiting Self-organization in Bioengineered Systems: A Computational Approach

Frontiers in Bioengineering and Biotechnology
Delin DavisNicholas S Flann

Abstract

The productivity of bioengineered cell factories is limited by inefficiencies in nutrient delivery and waste and product removal. Current solution approaches explore changes in the physical configurations of the bioreactors. This work investigates the possibilities of exploiting self-organizing vascular networks to support producer cells within the factory. A computational model simulates de novo vascular development of endothelial-like cells and the resultant network functioning to deliver nutrients and extract product and waste from the cell culture. Microbial factories with vascular networks are evaluated for their scalability, robustness, and productivity compared to the cell factories without a vascular network. Initial studies demonstrate that at least an order of magnitude increase in production is possible, the system can be scaled up, and the self-organization of an efficient vascular network is robust. The work suggests that bioengineered multicellularity may offer efficiency improvements difficult to achieve with physical engineering approaches.

References

Jan 1, 1965·Cold Spring Harbor Symposia on Quantitative Biology·J Adler
Apr 4, 2000·Biotechnology and Applied Biochemistry·A PandeyR Mohan
Nov 27, 2002·Biotechnology and Bioengineering·Anna Maria SandénGen Larsson
Oct 11, 2003·Biotechnology Advances·R SharmaU C Banerjee
Dec 8, 2004·Applied Microbiology and Biotechnology·Gary Walsh
Nov 15, 2005·Microbial Cell Factories·Bärbel Hahn-HägerdalWillem H van Zyl
Sep 15, 2006·Trends in Biotechnology·Mark A Eiteman, Elliot Altman
Aug 5, 1988·Biotechnology and Bioengineering·K Han, O Levenspiel
Aug 8, 2008·Microbial Cell Factories·Beate Pscheidt, Anton Glieder
Sep 20, 2008·PLoS Computational Biology·Roeland M H MerksJames A Glazier
Mar 17, 2009·Applied and Environmental Microbiology·Esben H HansenJørgen Hansen
Mar 25, 2009·Tissue Engineering. Part B, Reviews·Tamar KaullyShulamit Levenberg
Mar 26, 2009·Microbial Cell Factories·Neus Ferrer-MirallesAntonio Villaverde
Jun 6, 2009·Tissue Engineering. Part B, Reviews·Michael LovettDavid L Kaplan
Mar 17, 2010·Microscopy Research and Technique·Ignacio Arganda-CarrerasCarlos Ortiz-De-Solorzano
Jul 7, 2010·Microbial Cell Factories·Soniya Dhanjal, Swaranjit Singh Cameotra
Jul 7, 2010·Microbial Cell Factories·Antonio Villaverde
Nov 10, 2010·Microbial Cell Factories·Ana Rita BrochadoKiran Raosaheb Patil
Mar 18, 2011·Environmental Microbiology·Laurent A LardonBarth F Smets
Sep 14, 2011·Enzyme Research·Ramesh Chander KuhadAjay Singh
Jan 20, 2012·Nature·Daisy A Robinton, George Q Daley
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 28, 2012·Trends in Biotechnology·Abhinav A Shukla, Uwe Gottschalk
Dec 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·James N WilkingDavid A Weitz
Jan 15, 2013·Microbial Cell Factories·Jan Maarten van Dijl, Michael Hecker
Jul 17, 2013·Journal of Thrombosis and Haemostasis : JTH·R Monahan-EarleyW C Aird
Jul 30, 2014·Bioinformatics·Seunghwa KangIlya Shmulevich
Aug 26, 2014·Nature Chemical Biology·Kate ThodeyChristina D Smolke
Oct 18, 2014·Mathematical Medicine and Biology : a Journal of the IMA·R D O'DeaH M Byrne
Nov 27, 2014·Journal of Biomechanical Engineering·Kristen MorinRobert T Tranquillo
Mar 6, 2015·Bioresource Technology·Sujata Vijay SohoniPramod P Wangikar
Apr 19, 2015·Microbial Cell Factories·Shuchi H DesaiShota Atsumi
Jul 15, 2015·Molecular Reproduction and Development·Johannes SchindelinKevin W Eliceiri
Feb 11, 2016·Microbial Cell Factories·Laura Sanchez-GarciaAntonio Villaverde

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