Exploiting soft and hard X-ray absorption spectroscopy to characterize metallodrug/protein interactions: the binding of [trans-RuCl4(Im)(dimethylsulfoxide)][ImH] (Im = imidazole) to bovine serum albumin

Inorganic Chemistry
I AsconeAgostina Congiu Castellano

Abstract

The reaction of bovine serum albumin (BSA) with [ trans-RuCl 4(Im)(dimethylsulfoxide)][ImH] (Im = imidazole) (NAMI-A), an experimental ruthenium(III) anticancer drug, and the formation of the respective NAMI-A/BSA adduct were investigated by X-ray absorption spectroscopy (XAS) at the sulfur and chlorine K-edges and at the ruthenium K- and L 3-edges. Ruthenium K and L 3-edge spectra proved unambiguously that the ruthenium center remains in the oxidation state +3 after protein binding. Comparative analysis of the chlorine K-edge XAS spectra of NAMI-A and NAMI-A/BSA, revealed that the chlorine environment is greatly perturbed upon protein binding. Only modest changes were observed in the sulfur K-edge spectra that are dominated by several protein sulfur groups. Overall, valuable information on the nature of this metallodrug/protein adduct and on the mechanism of its formation was gained; XAS spectroscopy turns out to be a very suitable method for the characterization of this kind of systems.

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Citations

Dec 25, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·L MessoriA Congiu Castellano
Jul 16, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Michael I Webb, Charles J Walsby
Oct 12, 2017·Chemical Communications : Chem Comm·L Messori, A Merlino
Dec 10, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·V NovohradskýG Sava
Nov 1, 2009·Metallomics : Integrated Biometal Science·Aviva LevinaPeter A Lay
Nov 2, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Mani GaneshpandianMallayan Palaniandavar
Jan 7, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Michael I Webb, Charles J Walsby
Jul 28, 2016·Metallomics : Integrated Biometal Science·Gemma K GransburyHugh H Harris
Jan 31, 2013·Chemistry : a European Journal·Aviva LevinaPeter A Lay
Sep 2, 2009·Journal of the American Chemical Society·Thamayanthy SriskandakumarPierre Kennepohl

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