PMID: 7525988Dec 1, 1994Paper

Exploration of antigenic variation in gp120 from clades A through F of human immunodeficiency virus type 1 by using monoclonal antibodies

Journal of Virology
J MooreD D Ho

Abstract

The reactivities of a panel of 14 monoclonal antibodies (MAbs) with monomeric gp120 derived from 67 isolates of human immunodeficiency virus type 1 of clades A through F were assessed by using an antigen-capture enzyme-linked immunosorbent assay. The MAbs used were all raised against gp120 or gp120 peptides from clade B viruses and were directed at a range of epitopes relevant to human immunodeficiency virus type 1 neutralization: the V2 and V3 loops, discontinuous epitopes overlapping the CD4-binding site, and two other discontinuous epitopes. Four of the five V3 MAbs showed modest cross-reactivity within clade B but very limited reactivity with gp120s from other clades. These reactivity patterns are consistent with the known primary sequence requirements for the binding of these MAbs. One V3 human MAb (19b), however, was much more broadly reactive than the others, binding to 19 of 29 clade B and 10 of 12 clade E gp120s. The 19b epitope is confined to the flanks of the V3 loop, and these sequences are relatively conserved in clade B and E viruses. In contrast to the limited reactivity of V3 MAbs, CD4-binding site MAbs were much more broadly reactive across clades, two of these MAbs (205-46-9 and 21h) being virtually pan-reacti...Continue Reading

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Citations

Jun 10, 1997·AIDS Research and Human Retroviruses·J Moore, A Trkola
Oct 13, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·C J MasonS Nitayaphan
Dec 2, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·S LouisirirotchanakulJ Weber
Dec 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yongjun GuanGeorge K Lewis
Sep 29, 2015·The Journal of Clinical Investigation·Julia A M SungGuido Ferrari
Feb 26, 2005·Journal of Virology·Xinzhen YangJoseph Sodroski
Aug 3, 2004·Expert Review of Vaccines·Feng GaoBarton F Haynes
Nov 7, 2007·PLoS Pathogens·Meimei ShanJohn P Moore
Mar 29, 2014·Current Opinion in HIV and AIDS·George K LewisAnthony L Devico
Jan 31, 2017·Immunological Reviews·George K LewisAnthony L DeVico
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·S Karray, M Zouali
Nov 14, 1997·AIDS Research and Human Retroviruses·L ZhangD D Ho
Aug 2, 2013·Journal of Virology·Thaddeus M DavenportKelly K Lee

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