Exploration of Human Salivary Microbiomes--Insights into the Novel Characteristics of Microbial Community Structure in Caries and Caries-Free Subjects

PloS One
Jianye ZhouJizeng Wang

Abstract

Recently, high-throughput sequencing has improved the understanding of the microbiological etiology of caries, but the characteristics of the microbial community structure in the human oral cavity with and without caries are not completely clear. To better understand these characteristics, Illumina MiSeq high-throughput sequencing was utilized to analyze 20 salivary samples (10 caries-free and 10 caries) from subjects from the same town in Dongxiang, Gansu, China. A total of 5,113 OTUs (Operational Taxonomic Units, 97% cutoff) were characterized in all of the salivary samples obtained from the 20 subjects. A comparison of the two groups revealed that (i) the predominant phyla were constant between the two groups; (ii) the relative abundance of the genera Veillonella, Bifidobacterium, Selenomonas, Olsenella, Parascardovia, Scardovia, Chryseobacterium, Terrimonas, Burkholderia and Sporobacter was significantly higher in the group with caries (P < 0.05); and (iii) four genera with low relative abundance (< 0.01% on average), including two characteristic genera in caries (Chryseobacterium and Scardovia), significantly influenced the microbial community structure at the genus and OTU levels. Moreover, via co-occurrence and principal...Continue Reading

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Citations

Nov 30, 2016·International Journal of Molecular Sciences·Shan JiangEdward C M Lo
Dec 13, 2016·Applied and Environmental Microbiology·Jianye ZhouZhiqiang Li
Jan 2, 2019·Journal of Oral Microbiology·Reisha RafeekKaren E Nelson
Jul 21, 2017·Scientific Reports·Linda ErikssonIngegerd Johansson
May 14, 2020·Brazilian Oral Research·Naile Damé-TeixeiraThuy DO
Aug 3, 2019·Technology in Cancer Research & Treatment·Indranil ChattopadhyayMadhusmita Panda
Mar 5, 2020·Journal of Oral Microbiology·Daniel Belstrøm
Mar 20, 2018·Scientific Reports·Heidi Cecilie VillmonesØyvind Kommedal
Aug 4, 2021·Pediatric Research·Brittany K Wise-OringerAnne-Catrin Uhlemann

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Methods Mentioned

BETA
PCR
PCA

Software Mentioned

R
ggplots
LEfSe
R spaa package
Quantitative Insights Into Microbial Ecology ( QIIME )
BLAST
QIIME
EcoSim
R package vegan
HOMIM

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