Exploration of the hepatoprotective chemical base of an orally administered herbal formulation (YCHT) in normal and CCl4 -intoxicated liver injury rats. Part 2: Hepatic disposition in vivo and hepatoprotective activity in vitro

Journal of Ethnopharmacology
Xiao-Ting TianCheng-Gang Huang

Abstract

Yin-Chen-Hao Tang (YCHT) has been a very popular, hepatoprotective three-herb formula with an unclear chemical base. To reveal the hepatoprotective chemical base of oral-dosed YCHT, we bridged the hepatic disposition of six compounds in vivo and their hepatoprotection in vitro. In vivo, following the oral administration of YCHT in normal and CCl4-induced liver injury rats, the determinations of chlorogenic acid, 4-hydroxyacetophenone, geniposide, genipin, rhein and emodin were conducted in the portal vein plasma, the liver, and the systemic plasma. In vitro, the hepatoprotective activities of these compounds were determined in the CCl4-induced HepG2 cells. Consistent with the highest content in YCHT, geniposide had the highest exposure in vivo. Inconsistent with the negligible content, rhein, 4-hydroxyacetophenone, emodin and genipin showed substantial hepatic accumulations. In contrast, chlorogenic acid, an ingredient that has a high content in YCHT, elicited no hepatic exposure. In normal rats, the hepatic disposition prevented the compounds entering into the systemic plasma from the portal vein plasma by 44.9-100%, except for rhein. CCl4-induced liver injury caused a decreased hepatic exposure of 4-hydroxyacetophenone, rhein...Continue Reading

Citations

Aug 1, 2020·Frontiers in Pharmacology·Yibo HuQinghai Zeng
Jan 14, 2021·Journal of Applied Toxicology : JAT·Yitong LiuRobert L Sprando
Mar 2, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yahang WangYueming Ma
Nov 6, 2020·Journal of Ethnopharmacology·Raquel BridiChristian Espinosa-Bustos

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