Exploration of the potential roles of m6A regulators in the uterus in pregnancy and infertility.

Journal of Reproductive Immunology
Shisu ZhaoYulan Dong

Abstract

Infertility is a prevalent female reproductive disease worldwide. Currently, there are many unknown etiologies of infertility. N6-methyladenosine (m6A) is the most prevalent modification of eukaryotic mRNA. This study intended to investigate the implications of m6A regulators in the uterus for pregnancy and infertility. Pregnant ICR mice on days (D) 0, 4, 6, 10, and 15 were used to monitor m6A methylation in the uterus by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and then m6A methylation regulators were detected by real-time quantitative PCR (qPCR), western blot and immunohistochemistry (IHC). We found that m6A levels increased and that m6A regulators were expressed differently in the uterus during pregnancy. Then, we acquired expression data from endometrial tissue from women with infertility and recurrent pregnancy loss from the Gene Expression Omnibus (GEO) database. The expression of m6A regulators in infertility was significantly dysregulated according to the data mining technique. Specifically, the mRNA levels of METTL16 (p = 0.0147) and WTAP (p = 0.028) were lower and those of ALKBH5 (p = 0.0432) and IGF2BP2 (p = 0.0016) were higher in the endometrium of infertile patients. Meanwhile, many immunity-relat...Continue Reading

References

Aug 1, 1996·Human Molecular Genetics·A H McCannP A Dervan
Mar 1, 2008·Cytokine & Growth Factor Reviews·Wendy Chao, Patricia A D'Amore
Sep 30, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·S P SébertM E Symonds
May 10, 2012·Molecular Human Reproduction·Giulia GrimaldiJan J Brosens
May 12, 2012·Nature·Dan DominissiniGideon Rechavi
Dec 10, 2013·Nature Chemical Biology·Jianzhao LiuChuan He
Mar 13, 2014·Endocrinology·Tae Hoon KimJ Julie Kim
Sep 30, 2015·Stem Cells·Emma S LucasJan J Brosens
Jan 31, 2016·Molecular Human Reproduction·Ying QiaoEvica Rajcan-Separovic
Aug 16, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tie-Gang MengQing-Yuan Sun
Aug 2, 2019·Scientific Reports·Wanqing ZhaoYuan Zhou
Dec 6, 2019·Cell Death and Differentiation·Xiaoge HuLiu Yang
Dec 10, 2019·Cellular and Molecular Life Sciences : CMLS·Hong LiuAihua Liao
Jan 11, 2020·Advances in Medical Sciences·Marian Szamatowicz, Jacek Szamatowicz
Jan 23, 2020·Trends in Genetics : TIG·Yujin LeeYoon Ki Kim
Sep 9, 2020·Clinical and Translational Medicine·Xiaofeng SunHongguo Cao
Nov 23, 2020·Archives of Gynecology and Obstetrics·Xiaoyan SunXuehong Zhang
Nov 25, 2020·Aging·Li JiangXiaoling Fang

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