Exploration of the R code-based mathematical model for PMI estimation using profiling of RNA degradation in rat brain tissue at different temperatures

Forensic Science, Medicine, and Pathology
Jian-long MaLong Chen

Abstract

Precise estimation of postmortem interval (PMI) is crucial in some criminal cases. This study aims to find some optimal markers for PMI estimation and build a mathematical model that could be used in various temperature conditions. Different mRNA and microRNA markers in rat brain samples were detected using real-time fluorescent quantitative PCR at 12 time points within 144 h postmortem and at temperatures of 4, 15, 25, and 35 °C. Samples from 36 other rats were used to verify the animal mathematical model. Brain-specific mir-9 and mir-125b are effective endogenous control markers that are not affected by PMI up to 144 h postmortem under these temperatures, whereas the commonly used U6 is not a suitable endogenous control in this study. Among all the candidate markers, ΔCt (β-actin) has the best correlation coefficient with PMI and was used to build a new model using R software which can simultaneously manage both PMI and temperature parameters. This animal mathematical model is verified using samples from 36 other rats and shows increased accuracy for higher temperatures and longer PMI. In this study, β-actin was found to be an optimal marker to estimate PMI and some other markers were found to be suitable to act as endogenous...Continue Reading

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Citations

Dec 4, 2019·International Journal of Legal Medicine·Yehui LvYi-Jiu Chen
Dec 30, 2016·Forensic Science, Medicine, and Pathology·Ye-Hui LvLong Chen
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Jul 19, 2019·International Journal of Legal Medicine·Salvatore ScrivanoLuciana Caenazzo
Dec 31, 2020·Diagnostics·Anna RocchiIsabella Spinetti

Related Concepts

miR-125b, rat
MIRN9 microRNA, rat
N-Actin
Metazoa
Brain
Genetic Markers
Theoretical Study
Livor Mortis
Computer Programs and Programming
Specimen Collection

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