Exploratory analysis of quantitative histopathology of cervical intraepithelial neoplasia: objectivity, reproducibility, malignancy-associated changes, and human papillomavirus

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
M GuillaudC MacAulay

Abstract

As part of a project to evaluate emerging optical technologies for cervical neoplasia, our group is performing quantitative histopathological analyses of biopsy specimens from 1,190 patients. Objectives in the interim analysis are (a) quantitatively assessing progression of the neoplastic process of cervical intraepithelial neoplasia (CIN)/squamous intraepithelial lesions (SIL), (b) detecting malignancy-associated changes (MACs), and (c) phenotypically measuring human papillomavirus (HPV) detected by DNA testing. The diagnostic region of interest (ROI) from immediately adjacent sections were imaged, and the basal lamina and surface of the superficial layer were delimited. Nonoverlapping quantitatively stained nuclei were selected from 1,190 samples with histopathological characteristics of normal (929), koilocytosis (130), CIN 1 (40), CIN 2 (23), and CIN 3/carcinoma in situ (CIS) (68). A fully automatic procedure located and recorded the center of every nucleus in the region of interest (ROI). We used linear discriminant analysis to assess the changes between normal and CIN 3/CIS. Scores computed from the cell-by cell features and the clinical grade of CIN/SIL were highly correlated, as were those of the architectural features ...Continue Reading

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Citations

Apr 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Stephen LamCalum MacAulay
Apr 29, 2014·Computational and Mathematical Methods in Medicine·Ewert Bengtsson, Patrik Malm
Jul 27, 2010·Bio Systems·Martial GuillaudCalum Macaulay
Sep 18, 2007·Gynecologic Oncology·Clémence ThibervilleCalum MacAulay
Mar 3, 2007·Annals of the New York Academy of Sciences·Miriam P RosinCalum MacaUlay
Aug 30, 2008·Lasers in Surgery and Medicine·Chad A LieberAnita Mahadevan-Jansen
Oct 23, 2009·IEEE Transactions on Bio-medical Engineering·Dogan AltunbayCigdem Gunduz-Demir
Jun 12, 2013·Seminars in Cancer Biology·Attila Csikász-NagyMatteo Cavaliere
Jan 25, 2012·Journal of Biophotonics·Ivana K CecicCalum MacAulay
Jan 6, 2007·Cytometry. Part B, Clinical Cytometry·Michael E ScheurerKaren Adler-Storthz
Aug 1, 2014·Journal of the Royal Society, Interface·Emmanouil D ProtonotariosLewis D Griffin
Nov 21, 2019·Biomedizinische Technik. Biomedical Engineering·Christos KonstandinouGeorge Sakellaropoulos
Dec 2, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Calum MacAulayBranko Palcic
Oct 15, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hua TongUNKNOWN Mass Cervical Cancer Screening Regimen Group
Jun 8, 2010·Analytical Chemistry·Ronald O P DragaJ L H Ruud Bosch

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