Exploratory analysis of urinary tract infection using a GC-APCI-MS platform

The Analyst
Tiziana PacchiarottaOleg A Mayboroda

Abstract

Urinary tract infection (UTI) is among the most common bacterial infections worldwide. The understanding of the physiological mechanisms affected by UTI may need modern integrative '-omics' technologies, and metabolomics in particular. Here we present the first GC-APCI-MS-based explorative metabolomics study of UTI, using MS and FID detectors simultaneously. This provides high quality mass spectral data as well as semi-quantitative information demonstrating the feasibility of the GC-APCI-MS platform for non-targeted approaches. The work is part of a bigger project aiming at providing a comprehensive overview of UTI-induced changes in urine. Taking advantage of a fully clinically characterized cohort that offers the possibility of both case-control and longitudinal modelling, we can define UTI-induced change as a list of urinary metabolites which distinguish E. coli UTI patients from the subjects with no signs of an active infection. The list of molecular descriptors includes compounds related to bacterial activity such as lactic acid and lactose while other molecules show an association with the physiological status (inositol, citric acid).

References

Sep 18, 1980·The New England Journal of Medicine·D RudmanM H Kutner
Mar 1, 1997·The American Journal of Emergency Medicine·A G PinsonJ B Schorling
Jul 10, 2003·Infectious Disease Clinics of North America·Thomas M Hooton
Jul 18, 2003·The New England Journal of Medicine·Stephan D Fihn
Aug 1, 1954·The Journal of Clinical Investigation·W H DAUGHADAYE HOUGHTON
Oct 29, 2003·Archives of Internal Medicine·Calvin M Kunin
Apr 20, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Michael L Wilson, Loretta Gaido
May 20, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Gen SarashinaTakeshi Matsuoka
Aug 21, 2007·Journal of Proteome Research·Nikolaos G PsihogiosEleni T Bairaktari
Apr 28, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Cees van NieuwkoopJaap T van Dissel
Dec 9, 2010·Nature Reviews. Urology·Betsy Foxman
Feb 22, 2011·Molecular BioSystems·Crina I A BalogAndré M Deelder
Sep 2, 2011·Current Infectious Disease Reports·Willize E van der StarreCees van Nieuwkoop
Nov 15, 2011·Journal of Proteomics·Tiziana PacchiarottaOleg A Mayboroda
Feb 2, 2012·Journal of Proteome Research·Ashish GuptaMahendra Bhandari
Nov 9, 2012·Metabolomics : Official Journal of the Metabolomic Society·Ekaterina NevedomskayaAndré M Deelder
Jun 26, 2013·Bioanalysis·Tiziana PacchiarottaOleg A Mayboroda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Metabolomics : Official Journal of the Metabolomic Society
Ekaterina NevedomskayaAndré M Deelder
Comptes rendus des séances de la Société de biologie et de ses filiales
Y DUPUIS
Biomedizinische Technik. Biomedical Engineering
Oana A TomescuGerhard G Thallinger
© 2022 Meta ULC. All rights reserved